EXPERIMENTAL STUDY ON THE TREATMENT OF RAT ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS WITH MANNITAL_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

Li Li ¹ , Liu Chengxun ¹ , Zhang Qiang ² , et al. ²

The People’s Hospital of Liaoning Province, Shenyang 110015;

Corresponding author：

Keywords：

Oxygen-derived free radical; Pancreatitis; Mannital; Rat

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This study aimed to assess the therapeutic effect of mannital administration on acute hemorrhagic necrotizing pancreatitis (AHNP), 28 Wistar rats were randomily divided into therapeutic group and control group after induction of AHNP by retrograde intraductal injection of 5 percent sodium taurocholate. The rats of therapeutic group received intravenous 20% mannital (1g/kg) through tail vein, once in 12 hours, until the end of experiment; control group received saline (5.0 ml/kg) with the same way. Blood of all the rats were collected from heart and the rats were killed after 96 hours. Results: lipid peroxide (LPO) in pancreatic tissue, LPO in serum, lactate dehydrogenase (LDH), alpha-1-antitrypsin (α1-AT), glutamicoxalacetic transaminase (GOT), necrotizing square of pancreatic tissue in the therapeutic group were significantly less than those in control group (Ｐ＜0.05 or Ｐ＜0.01). The damage to pancrease, heart, liver, kidney in the therapeutic group were lighter than those of the control group and the mortality was lower (Ｐ＜0.05).Conclusions: Mannital can scavenge the oxygenderived free radicals and play a therapeutic role in AHNP.

Citation： Li Li,Liu Chengxun,Zhang Qiang,et al.. EXPERIMENTAL STUDY ON THE TREATMENT OF RAT ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS WITH MANNITAL. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(6): 332-334. doi: Copy

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1. Beger HG. Surgical management of necrotizing pancreatitis. Surg Clin of North AM, 1989; 69∶529.
2. Gough DB, Boyle B, Joyce WP, et al. Free radical inhibition and serial chemiluminescence in evolving experimental pancreatitis. Br J Surg, 1990; 77∶1256.
3. Furukawa M. Role of oxygenderived free redicals in acute necrotizing pancreatitis induced by stress and cerulein in rats. Pancreas, 1994; 9(1)∶67.
4. Sanfey H, Bulkley GB, Cameron JL. The role of oxygenderived free radicals in pathogenesis of acute pancreatitis. Ann Surg, 1984; 200(4)∶405.
5. Michael D, Cavarocchi NC, Obrien JF. Influence of antioxidants (mannitol and allopurinol) on oxygen free radical generation during and after cardiopulmonary bypass. Circulation, 1986; 74(Suppl Ⅲ)∶134.
6. Johnson RM, Barone RM, Newson Bl, et al. Treatment of experimental acute pancreatitis with 5Fluorouracil. Am J Surg, 1973; 125(2)∶211.
7. Chardavoyne R, Asher A, Bank S, et al. Role of reactive oxygen metabolites in early cardiopulmonary changes of acute hemorrhagic pancreatitis. DigDisSci, 1989; 34∶1581.
8. 丁长海.抗氧自由基药物研究进展.中国药理学通报，1992；8(5)∶321.
9. Wang ZH, Lguchi H, Ohshio G, et al. Increased pancreatic metallothionein and glutathione levels: protecting against cerulein and taurocholate induced acute pancreatitis in rats. Pancreas, 1996; 13(2)∶173.
10. Hirano T, Furuyama H, Kawakami Y, et al. Protective effect of prophylaxis with a protease inhibitor and a free radical scavenger against a temporary ischemia model of pancreatitis. Canadian Journal of Surgery, 1995; 38(3)∶241.
11. Schoenberg MH, Buchler M, Younes M, et al. Effect of antioxidant treatment in rats with acute hemorrhagic pancreatitis. DigDisSci, 1994; 39(5)∶1034.
12. Closa D, Hotter G, Carafan J, et al. Prostanoids and oxygen free radicals in early stages of experimental pancreatitis. DigDisSci, 1994; 39(7)∶1537.
13. 蒋昆，鲍耀东，阮长耿.自由基清除剂缓解oxyHb的致脑血管痉挛的实验研究.中华神经外科杂志，1989； 5(2)∶101.
14. 安梅，李主一，翁龙江.甘露醇对肢体缺血再灌注所致急性肺损伤的防治作用的研究.中国危重急救医学，1994；6(1)∶7.
15. 李宏，伍贻经，时安云.活性氧对心肌细胞膜ATP酶活性的影响.中国病理生理杂志，1990； 6(4)∶197.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

EXPERIMENTAL STUDY ON THE TREATMENT OF RAT ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS WITH MANNITAL

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