A ANIMAL MODEL FOR STUDY OF THE AGGRAVATING MECHANISM OF ACUTE PANCREATITIS_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041;

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Keywords：

Experimental pancreatitis; Calcium overloading; Animal model

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To introduce a rat model of the conversion of acute edematous pancreatitis (AEP) to necrotizing pancreatitis (ANP). One hundred and seven Sprague-Dawley rats were randomized in three experimental groups as follows: sham operation control group and AEP group and ANP group. AEP was induced by pancreatic duct ligation and exocrine stimulation, ANP was induced same as AEP，but with a large dose of dextran-110 (500mg/kg) intravenously. The serum concentration of amylase increased significantly in AEP group and ANP group. Cytosolic free Ca2+ concentration in isolated pancreatic acinar cells increased consistently after induction of ANP. Homorrhage, parenchymal necrosis and calcium deposits in acinar cells were observed in pancreas in ANP group. Ultrastructural examination showed desquamation and necrosis of the endothelium of the pancreatic capillary in ANP group. These results suggest that ischemia may induce the conversion of AEP to ANP via acinar cell Ca2+ overloading. The rat model would seem to be a suitable animal model for studying aggravating mechanism of acute pancreatitis.

Citation： Pu Qingfan,Yan Lnan,Liu Xubao,et al.. A ANIMAL MODEL FOR STUDY OF THE AGGRAVATING MECHANISM OF ACUTE PANCREATITIS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 1998, 5(6): 335-337. doi: Copy

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1. Yan LN, Liu XB, Tan JS, et al. Effect of haemorrheological changes in the transition of oedematous pancreatitis to necrosis. Asian J Surgery, 1995; 18(2)∶106.
2. Zhou WG, Levine BA, Olson MS. Pateletactivating fator: a mediator of pancreatic inflammation during cerulein hyperstimulation. Am J Pathol, 1993; 142(5)∶1504.
3. Kyogoku T, Manabe T, Tobe T. Role of ischemia in acute pancreatitis: Hemorrhagic shock converts edematous pancreatitis to hemorrhagic pancreatitis in rats. Dig Dis Sci, 1992; 37(9)∶1409.
4. Prinz RA. Mechanisms of acute pancreatitis: vascular etiology. Int J Pancreatol, 1991; 9∶31.
5. Bockman DE. Microvasculature of the pancreas relation to pancreatitis. Int J Pancreatol, 1992; 12(1)∶11.
6. Schulz HU, Letko G, Spormann H. Decreased survivial rate of pancreatic acinar cells isolated from rats with acute pancreatitis. In J Pancreatol, 1990; 6∶219.
7. Ochs DL, Korenbrot JI, Williams JA. Relation between free cytosolic calcium and amylase release by pancreatic acini. Am J Physiol, 1985; 249∶G389.
8. Closa D, Hotter G, Bulbena O, et al. Calcium channel blockers in experimental acute pancreatitis. Effect on tissue prostanoids and oxygen free radicals. Pancreas, 1996; 12(2)∶178.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

A ANIMAL MODEL FOR STUDY OF THE AGGRAVATING MECHANISM OF ACUTE PANCREATITIS

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