Effects of FasL gene-modified dendritic cell on the airway inflammation in mice sensitized/challenged by house dust mite allergen_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Yan ¹ , ZHUO Wenlei ² , BI Yutian ¹ , WU Kui ¹ ,  WANG Changzheng ¹ , SONG Yunxi ¹ , XIA Junbo ¹

Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University.Chongqing，400037，ChinaCorresponding author：WANG Chang-zheng，E-mail：czwang@netease.com;

Corresponding author：

WANG Changzheng, Email: czwang@netease.com

Keywords：

FasL gene; Dendritic cell; Allergic airway inflammation

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Objective To investigate the effects of FasL gene-modified dendritic cell (DC) on the airway inflammation in mice sensitized/challenged by house dust mite (HDM) allergen.Methods FasL gene-modified DC (FasL-DC) and control DC (nontransfection DC) were administrated into HDM sensitized and challenged mice by intratracheal injection respectively，then HDM sensitized and challenged mice were sacreificed two days later.Total and differentiation cell counts and levels of interleukin-4（IL-4），IL-5 and interferon-γ(IFN-) in bronchoalveolar lavage fluid (BALF) were detected and lung histological features were observed.Results After administration of FasL-DC，lung allergic inflammation was ameliorated while total cell counts，the percentage of eosinophil ,the levels of IL-4 and IL-5 in BALF decreased and the level of IFN- in BALF increased.Conclusion Administrating FasL-DC into HDM sensitized/challenged mice can inhibit Th2 cells activation and ameliorate airway allergic inflammation.

Citation： WANG Yan,ZHUO Wenlei,BI Yutian,WU Kui,WANG Changzheng,SONG Yunxi,XIA Junbo. Effects of FasL gene-modified dendritic cell on the airway inflammation in mice sensitized/challenged by house dust mite allergen. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(1): 15-18. doi: Copy

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12.	Zhang HG，Su X，Liu D，et al.Induction of specific T cell tolerance by Fas ligand-expressing antigen presenting cells.J Immunol，1999，162：1423-1430.
13.	Zhan HG，Mountz JD，Fleck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，Fas ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
14.	Zhang HG，Liu D，Heike Y，et al.Induction of specific T-cell tolerance by adenovirus-transfected，Fas ligand-producing antigen presenting cells.Nat Biotechnol，1998，16：1045-1049.
15.	Matsue H，Matsue K，Walters M，et al.Induction of antigen-specific immunosuppression by CD95L cDNA-transfected ’killer’ dendritic cells.Nat Med，1999，5：930-937.
16.	Kusuhara M，Matsue K，Edelbaum D，et al.Killing of naïve T cells by CD95L-transfected dendritic cells(DC)：in vivo study using killer DC-DC hybrids and CD4(+) T cells from DO11.10 mice.Eur J Immunol，2002，32：1035-1043.

1. Suda T，Takahashi T，Golstein P，et al.Molecular cloning and expression of the Fas ligand，a novel member of the tumor necrosis factor family.Cell，1993，75：1169-1178.
2. Bohana-Kashtan O，Civin CI.Fas ligand as a tool for immunosuppression and generation of immune tolerance.Stem Cells，2004，22：908-924.
3. Griffith TS，Brunner T，Fletcher SM，et al.Fas ligand-induced apoptosis as a mechanism of immune privilege.Science，1995，270：1189-1192.
4. Askenasy N，Yolcu ES，Yaniv I，et al.Induction of tolerance using Fas ligand：a double-edged immunomodulator.Blood，2005，105：1396-1404.
5. Quah BJ，O’Neill HC.Maturation of function in dendritic cells for tolerance and immunity.J Cell Mol Med，2005，9：643-654.
6. Zhang HG，Mountz JD，Fleck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，Fas ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
7. 夏俊波，吴奎，孙鲲，等.小鼠骨髓来源的树突状细胞体外扩增与鉴定.第三军医大学学报，2005，27：942-944.
8. Hansen G，Berry G，DeKruyff RH，et al.Allergen-specific Th1 cells fail to counterbalance Th2 cell-induced airway hyperreactivity but cause severe airway inflammation.J Clin Invest，1999，103：175-183.
9. Dahl ME，Dabbagh K，Liggitt D，et al.Viral-induced T helper type 1 responses enhance allergic disease by effects on lung dendritic cells.Nat Immunol，2004，5：337-343.
10. Chuang YH，Fu CL，Lo YC，et al.Adenovirus expressing Fas ligand gene decreases airway hyper-responsiveness and eosinophilia in a murine model of asthma.Gene Ther，2004，11：1497-1505.
11. Min WP，Gorczynski R，Huang XY，et al.Dendritic cells genetically engineered to express Fas ligand induce donor-specific hyporesponsiveness and prolong allograft survival.J Immunol，2000；164：161-167.
12. Zhang HG，Su X，Liu D，et al.Induction of specific T cell tolerance by Fas ligand-expressing antigen presenting cells.J Immunol，1999，162：1423-1430.
13. Zhan HG，Mountz JD，Fleck M，et al.Specific deletion of autoreactive T cells by adenovirus-transfected，Fas ligand-producing antigen-presenting cells.Immunol Res，2002，26：235-246.
14. Zhang HG，Liu D，Heike Y，et al.Induction of specific T-cell tolerance by adenovirus-transfected，Fas ligand-producing antigen presenting cells.Nat Biotechnol，1998，16：1045-1049.
15. Matsue H，Matsue K，Walters M，et al.Induction of antigen-specific immunosuppression by CD95L cDNA-transfected ’killer’ dendritic cells.Nat Med，1999，5：930-937.
16. Kusuhara M，Matsue K，Edelbaum D，et al.Killing of naïve T cells by CD95L-transfected dendritic cells(DC)：in vivo study using killer DC-DC hybrids and CD4(+) T cells from DO11.10 mice.Eur J Immunol，2002，32：1035-1043.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of FasL gene-modified dendritic cell on the airway inflammation in mice sensitized/challenged by house dust mite allergen

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