Safety study of intravenous infusion with self-made perfluorocarbon emulsion in rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

FAN Haojun ¹ , LIU Shuying ² ,  ZHANG Jianpeng ¹ , LIU Youning ³

Department of Respiratory Medicine，General Hospital of Chinese People`s Armed Police Forces.Beijing 100039，ChinaCorresponding Author：ZHANG Jian-peng;;
E-mail：zjp99@vip.sina.com;

Corresponding author：

ZHANG Jianpeng, Email: zjp99@vip.sina.com

Keywords：

Perfluorocarbon; Emulsion; Intravenous infusions; Safety study

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Objective To assess the safety of intravenous infusion with self-made perfluorocarbon emulsion (PFE) in rats.Methods 35 Wistar rats were randomly divided into a normal control group and six PFE groups (n=5 in each group).The control group was intravenous injected with normal saline and six PFE groups with PFE.Blood samples were obtained from the PFE groups 5 min after injection via vena orbitalis for perfluorocarbon (PFC) measurement.The control group were sacrificed at 2 hours after injection，and the six PFE groups were respectively killed at 2 h，4 h，6 h，24 h，48 h and 10 d after injection.The blood levels of PFC，PaO2，GPT，GOT，BUN and Cr were measured.Pathological changes of lung，liver and kidney were also observed.Results All rats were in good health state after PFE intravenous injection with no obvious pathological changes in liver and kidney.PFC was wide distributed throughout the pulmonary alveoli and capillaries.The levels of GPT and GOT were higher in the PFC groups at 2，4，6 and 24 h than which in the control group (all P lt;0.05)，but there were no significant difference between the PFE 10 d group and the control group.The levels of BUN and Cr had no remarkably differences in all groups.Blood PFC concentrations were (20±1.8)mg/mL，(1.8±0.7)mg/mL，(1.5±0.6)mg/mL，(1.2±0.4)mg/mL，(0.5±0.2)mg/mL，(0.2±0.03)mg/mL，0 mg/mL in the PFE groups respectively at 5 min，2 h，4 h，6 h，24 h，48 h，10 d after injection.PaO2 of the PFE 2 h group was significant higher than which in the control group [(119.2±8.6)mm Hg vs (99.6±4.7)mm Hg，P lt;0.05].Conclusion Intravenous injection with PFE (10 mL/kg) can enhance PaO2 with good safety and no obvious influence on pathology of lung，liver and kidney，so may be used for the treatment study of acute lung injury.

Citation： FAN Haojun,LIU Shuying,ZHANG Jianpeng,LIU Youning. Safety study of intravenous infusion with self-made perfluorocarbon emulsion in rats. Chinese Journal of Respiratory and Critical Care Medicine, 2007, 6(2): 123-126. doi: Copy

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1. Lowe KC.Second-generation perfluorocarbon emulsion blood substitutes.Artif Cells Blood Substit Immobil Biotechnol，2000，28：25-38.
2. 葛文正，曹文娟.氟碳代血液.生物学通报，1986，21：20-22.
3. Leese PT.，Noveck RJ，Shorr JS，et al.Randomized safety studies of intravenous perflubron emulsion.I.Effects on coagulation function in healthy volunteers.Anesth Analg，2000；91：804-811.
4. 樊毫军，张健鹏，刘又宁.吸入全氟化碳治疗急性肺损伤研究进展.中国呼吸与危重病监护杂志，2006，5：63-65，45.
5. 张健鹏，刘又宁.何谓液体通气？有无临床应用前景？中华结核和呼吸杂志，2002，25：143-145.
6. Gould SA，Rosen AL，Sehgal LR，et al.Fluosol-DA as a red-cell substitute in acute anemia.N Eng J Med，1986，314：1653-1656.
7. Mathurin JC，Ceaurriz J，Audran M，et al.Detection of perfluorocarbons in blood by headspace solid-phase microextraction combined with gas chromatography/mass spectrometry.Biomed Chromatogr，2001，15：443-451.
8. Papadimitriou DK，Pitoulias GA，Kotakidou RE，et al.Prolongation of intestineal viability using oxygenated perfluorocarbon in an experimental model of acute intestineal ischemia.Eur J Vasc Endovasc Surg，2004，28：636-641.
9. Koch T，Ragaller M，Haufe D，et al.Perfluorohexane attenuates proinflammatory and procoagulatory response of activated monocytes and alveolar macrophages.Anesthesiology，2001，94：101-109.
10. Hirayama Y，Hirasawa H，Oda S，et al.Partial liquid ventilation with FC-77 suppresses the release of lipid mediators in rat acute lung injury model.Crit Care Med，2004，32：2085-2089.
11. Spahn DR，Pasch T.Physiological properties of blood substitutes.News Physiol Sci，2001，16：38-41.
12. Nader ND，Knight PR，Davidson BA，et al.Systemic perfluorocarbons suppress the acute lung inflammation after gastric acid aspiration in rats.Anesth Analg，2000，90：356-361.
13. Reickert C，Pranikoff T，Overbeck M，et al.The pulmonary and systemic distribution and elimination of perflubron from adult patients treated with partial liquid ventilation.Chest，2001，119：515-522.
14. Daugherty WP，Levasseur JE，Sun D，et al.Perfluorocarbon emulsion improves cerebral oxygenation and mitochondrial function after fluid percussion brain injury in rats.Neurosurgery，2004，54：1223-1230.

Chinese Journal of Respiratory and Critical Care Medicine

Safety study of intravenous infusion with self-made perfluorocarbon emulsion in rats

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