Effect of wasabi on the expression of hnRNP A2/B1 in lung cancer_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

TAO Ran ,  LI Weimin , CHEN Wenbin

Department of Respiratory Medicine ， West China Hospital ， Sichuan University.Chengdu ， Sichuan ， 610041 ， Chinac orresponding author：LI Wei-min，E-mail：weimi003@yahoo.com;

Corresponding author：

LI Weimin, Email: weimi003@yahoo.com

Keywords：

Wasabi; Rat; 3-methylcholanthrene; Lung cancer; HnRNP A2/B1

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Objective An animal model of lung cancer was established to study whether wasabi could inhibit the expression of hnRNP A2/B1 in lung.Methods Thirty-six Wistar rats were randomly divided as model group and wasabi group.0.1 mL of arcinogenic iodized oil [50 mg 3-methylcholanthrene (MCA) in 1 mL carcinogenic iodized oil] were instilled intratracheally to induce lung cancer.A week before instillation of MCA，the wasabi group was orally administered wasabi extract solution until the animals were killed while the model group was given isometric saline at the same time.Six rats in each group were randomly killed on 30th day，60th day and 90th day.Immunohistochemisty and RT-PCR were used to measure the protein and mRNA expression of hnRNP A2/B1，respectively.Results Wasabi lowered the protein expression of hnRNP A2/B1 with a total inhibitory rate of 48.5%.At the 30th，60th and 90th day，the inhibitory rate was 51.0%，51.0% and 45.1% respectively.Meanwhile，wasabi lowered the mRNA expression of hnRNP A2/B1 with a total inhibitory rate of 60.5%.At the 60th and 90th day，the inhibitory rate was 79.5% and 58.0%，respectively.Conclusion Wasabi solution can down-regulate the expression of hnRNP A2/B1 which may be a molecular mechanisms by which wasabi inhibits lung cancer.

Citation： TAO Ran,LI Weimin,CHEN Wenbin. Effect of wasabi on the expression of hnRNP A2/B1 in lung cancer. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(2): 107-109. doi: Copy

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1. 杨艳,曹箭.肺癌早期诊断相关标记物的研究进展.诊断病理学杂志,2007,14:229-231.
2. Genoveva Murillo,Rajjendra G Mehta.Cruciferous Vegetables and Cancer Prevention.Nutrition Cancer,2001,41:17-28.
3. Cohen JH,Kristal AR,Stanford JL.Fruit and vegetable intakes and prostate cancer risk.J Natl Cancer Inst,2000,92:61-68.
4. Watanabe M,Ohata M,Hayakawa S,et al.Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi.Phytochemistry,2003,62:733-739.
5. Morimitsu Y,Nakagawa Y,Hayashi K,et al.A sulforaphane analogue that potently activates the Nrf2-dependent detoxification pathway.J Biol Chem,2002,277:3456-3463.
6. 田鸿生,刘铭球,高文琴,等.建立动物肺癌模型的方法学研究——Ⅱ.肺叶支气管内灌注致癌质碘油溶液诱发大白鼠肺癌的新方法.肿瘤防治研究,1982,9:2-4.
7. 孙敬方.动物实验方法学.北京:人民卫生出版社,2004,308-309.
8. 黄明深.山葵调味品原料中异硫氰酸酯的含量及影响因素研究.中国调味品,2005,6:21-23.
9. Yano T,Yajima S,Virgona N,et al.The effect of 6-methylthiohexyl isothiocyanate isolated from Wasabia japonica (wasabi) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-buatnone-induced lung tumorigenesis in mice.Cancer Lett,2000,155:115-120.
10. Tockman MS,Mulshine JL,Piantadosi S,et al.Prospective detection of preclinical lung cancer:results from two studies of heterogeneous nuclear ribonucleoprotein A2/B1 overexpression.Clin Cancer Res,1997,3:2237-2246.
11. Fujimoto N,Sueoka N,Sueoka E,et al.Lung cancer prevention with(-)-epigallocatechin gallate using monitoring by heterogeneous nuclear ribonucleoprotein B1.Int J Oncol,2002,20:1233-1239.
12. Garayoa M,Man YG,Martinez A,et al.Downregulation of hnRNP A2/B1 expression in tumor cells under prolonged hypoxia.Am J Respir Cell Mol Biol,2003,28:80-85.

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Effect of wasabi on the expression of hnRNP A2/B1 in lung cancer

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