Effects of dexamethasone on the expression of CCL1 and CCR8 in asthma mice_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

WANG Zeyu ,  LIU Jianbo

Department of Respiration Medicine，The Second Affiliated Hospital of Zhengzhou University.Zhengzhou，Henan,450014，ChinaCorresponding Author：LIU Jian-bo，E-mail：jbliuzz@yahoo.com.cn;

Corresponding author：

LIU Jianbo, Email: jbliuzz@yahoo.com.cn

Keywords：

Asthma; CC chemokine ligand-1; CC chemokine receptor-8; Interlukin-4; Dexamethasone

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Objectives To observe the expression of CCL1/CCR8 mRNA in murine lung tissue of bronchial asthma and effects of glucocorticoids on their expression. Methods Thirthy healthy mice were randomly divided into a control group, an asthma group, and a dexamethasone group, with 10 mice in each group. The sensitized murine asthma model was induced by ovalbumin sensitization and challenge, and the dexamethasone group were peritoneally injected with dexamethasone( 2 mg/ kg) . Total and differential cell counts in BALF were measured. IL-4 Level in BALF was evaluated by ELISA. The expression of CCL1 and CCR8 mRNA in the lungs were measured by semi-quantitative RT-PCR. Results The percentage of eosinophils, lymphocyte and IL-4 level in the asthma group increased significantly compared with the control
group, and which in the dexamethasone group decreased significantly compared with the asthma group and still higher than the control group( all P lt; 0. 01) . The expression of CCL1 and CCR8 mRNA had the same tendency ( all P lt;0. 01) . Conclusions The gene expression of CCL1/CCR8 is up-regulated in allergic asthma mice.
Glucocorticoids can relieve airway inflammation of asthma probably by inhibiting CCL1/CCR8 expression.

Citation： WANG Zeyu,LIU Jianbo. Effects of dexamethasone on the expression of CCL1 and CCR8 in asthma mice. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(1): 32-35. doi: Copy

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10.	Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
11.	Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
12.	李锋,刘荣玉.气道炎症和重构在小鼠哮喘模型中的变化及地塞米松的干预作用。中国呼吸与危重监护杂志.2005,4:110-114.

1. Heijink IH,Van Oosterhout AJ.Targeting T cells for asthma.Curr Opin Pharmacol,2005 ,5:227-231.
2. Zingoni A,Soto H,Hedrick JA,et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells.J Immunol,1998,161:547-551.
3. Gombert M, Dieu-Nosjean MC, Winterberg F,et al. CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. J Immunol, 2005,174:5082-5091.
4. Cantor J, Haskins K. Recruitment and activation of macrophages by pathogenic CD4 T cells in type 1 diabetes: evidence for involvement of CCR8 and CCL1.J Immunol,2007,79:5760-5767.
5. Larché M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol,2003,111:450-463.
6. Laudanna C, Kim JY, Constantin G,et al. Rapid leukocyte integrin activation by chemokines. Immunol Rev,2002,186:37-46.
7. Moser B, Loetscher M,Piali L,et al.Lymphocyte responses to chemokines.Int Rev Immunol, 1998,16:323-344.
8. Gonzalo JA,Qiu Y,Lora JM,et al.Coordinated involvement of mast cells and T cells in allergic mucosal inflammation:critical role of the CC chemokine ligand 1:CCR8 axis.J Immunol, 2007,179:1740-1750.
9. Chensue SW,Lukacs NW,Yang TY,et al.Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J Exp Med, 2001,193:573-584.
10. Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
11. Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
12. 李锋,刘荣玉.气道炎症和重构在小鼠哮喘模型中的变化及地塞米松的干预作用。中国呼吸与危重监护杂志.2005,4:110-114.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of dexamethasone on the expression of CCL1 and CCR8 in asthma mice

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