Nitric Oxide Inhalation Alleviates Lung Inflammation of Rats with Acute Lung Injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHANG Jianchu , BAI Ming , WU Yanwen , ZHANG Lei , BAO Bin.

Department of Respiratory Medicine, Union Hospital of Tongji Medical College, Huazhong University of Science &;
Technology. Wuhan, Hubei,430022, ChinaCorresponding Author: ZHANG Jian-chu, E-mail: zsn0928@ 163. com;

Corresponding author：

Keywords：

Acute lung injury; Nitric oxide; Toll-like receptor 4; Interleukin-6

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Objective To observe the effects of nitric oxide ( NO) inhalation on lung inflammation of acute lung injury ( ALI) in rats. Methods Twenty-four SD rats were randomly divided into four groups, ie. a normal control group, an ALI group, a 20 ppm NO inhalation group, and a 100 ppm NO inhalation group. ALI model was established by LPS instillation intratracheally and the control group was instilled with normal saline. Then they were ventilated with normal air or NO at different levels, and sacrificed 6 hours later. Pathological changes were evaluated by HE staining. The expression of TLR4 in lung tissues was detected by immunohistochemistry. IL-6 level in lung homogenate was measured by ELISA. Results In the ALI group, the inflammation in bronchus and bronchioles was more apparently, and the expressions of TLR4
and IL-6 were elevated significantly compared with the control group. 20 ppm NO inhalation significantly decreased the expression of TLR4 and IL-6, and alleviated the inflammation of ALI. However, 100 ppm NO inhalation did not change TLR4 expression and lung inflammation significantly, and increased IL-6 level.Conclusions Inhalation low level of NO( 20 ppm) can alleviate lung inflammation possibly by reducing the
expression of TLR4 and IL-6.

Citation： ZHANG Jianchu,BAI Ming,WU Yanwen,ZHANG Lei,BAO Bin.. Nitric Oxide Inhalation Alleviates Lung Inflammation of Rats with Acute Lung Injury. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(2): 150-153. doi: Copy

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7.	邱海波, 杨毅, 周韶霞, 等. 吸入不同浓度一氧化氮对急性肺损伤小鼠肺组织炎症反应的影响. 江苏医药杂志, 2004 , 30: 894 -897.
8.	赵玮, 王砚, 唐法娣, 等. LPS 致大鼠急性肺损伤TLR4 的表达及蓝桉油的影响. 中国中药杂志, 2006, 31: 319 -322.
9.	朱桂军, 刘俊峰, 于占彪, 等. 二烯丙基三硫对急性肺损伤小鼠肿瘤坏死因子-α表达及核转录因子-κB 活性的影响. 中国危重病急救医学杂志, 2007, 19: 205 -208.
10.	Bone RC. The pathogenesis of sepsis. Ann Intern Med, 1991, 115 :457-469.
11.	Fattori E, Cappelletti M, Costa P, et al. Defective inflammatory response in interleukin 6-deficient mice. J Exp Med, 1994 , 180 :1243-1250.
12.	Spiecker M, Darius H, Koboth K, et al. Differential regulation of endothelial cell adhesion molecule expression by nitric oxide donors and antioxidants. J Leukoc Biol, 1998 , 63 : 732 -739.

1. Bernard GR, Artigas A, Brigham KL, et al. Report of the American-European consensus conference on ARDS: definitions, mechanisms,relevant outcomes and clinical trial coordination. The consensus Committee. Intensive Care Med, 1994, 20 : 225-232.
2. Poltorak A, He X, Smirnova I, et al. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in TLR4 gene.Science, 1998 , 282: 2085.
3. Chow J, Young DW, Golenbock DT, et al. Toll-like receptor-4 mediates lilpopolyssccharide-induced signal transduction. J Bio Chem, 1999, 274 : 10689-10692 .
4. Kang JL, Park W, Pack IS, et al. Inhaled nitric oxide attenuates acute lung injury via inhibition of nuclear factor-kappa B and inflammation. J Appl Physiol, 2002, 92: 795-801 .
5. 郭振辉, 邹霞英, 樊满齐, 等. 一氧化氮吸入治疗急性肺损伤的作用机制. 中华实验外科杂志, 2001, 18: 556-558 .
6. 杨毅, 邱海波, 周韶霞, 等. 吸入一氧化氮对急性肺损伤小鼠肺组织炎症反应的影响. 中国危重病急救医学杂志, 2002 , 14 :723-727.
7. 邱海波, 杨毅, 周韶霞, 等. 吸入不同浓度一氧化氮对急性肺损伤小鼠肺组织炎症反应的影响. 江苏医药杂志, 2004 , 30: 894 -897.
8. 赵玮, 王砚, 唐法娣, 等. LPS 致大鼠急性肺损伤TLR4 的表达及蓝桉油的影响. 中国中药杂志, 2006, 31: 319 -322.
9. 朱桂军, 刘俊峰, 于占彪, 等. 二烯丙基三硫对急性肺损伤小鼠肿瘤坏死因子-α表达及核转录因子-κB 活性的影响. 中国危重病急救医学杂志, 2007, 19: 205 -208.
10. Bone RC. The pathogenesis of sepsis. Ann Intern Med, 1991, 115 :457-469.
11. Fattori E, Cappelletti M, Costa P, et al. Defective inflammatory response in interleukin 6-deficient mice. J Exp Med, 1994 , 180 :1243-1250.
12. Spiecker M, Darius H, Koboth K, et al. Differential regulation of endothelial cell adhesion molecule expression by nitric oxide donors and antioxidants. J Leukoc Biol, 1998 , 63 : 732 -739.

Chinese Journal of Respiratory and Critical Care Medicine

Nitric Oxide Inhalation Alleviates Lung Inflammation of Rats with Acute Lung Injury

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