Experimental Study on the Relation of Mucosal Immunity in Asthma Rats’Lung,Nose and Intestine_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

LI Fengsen ¹ , QINHuijuan ² , HAMULATI ¹

Chinese Medical Hospital of Xinjiang Medical University. Urumqi,Xinjiang, 830000, ChinaCorresponding Author: QIN Hui-juan, E-mail: qin0827@ 163. com;

Corresponding author：

Keywords：

Asthma; Mucosal immunity; Lymphocyte; Eotaxin

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the relevance and changes of mucosal immunity in asthma rats’lung, nose and intestine. Methods Twenty Wistar rats were randomly divided into a normal group and an asthma group. Asthma rat model was established by sensitization and challenge with ovalbumin. CD4 + ,CD8 + , eotaxin protein and its mRNA in rats’lung tissues, rhinal and intestinal mucosa were measured by immunohistochemical methods and situ hybridization. The content of sIgA in bronchoalveolar lavage fluid ( BALF) , nasopharyngeal washings and intestinal mucus supernatant were detected by enzyme-linked immunosorbent assay. Results Compared with the normal group, the levels of CD4 + , CD8 + in rats’lung tissues, rhinal and intestinal mucosa, the expression of eotaxin protein and mRNA in rats’lung tissues, the content of sIgA in nasopharyngeal washing, and the expression of eotaxin protein in intestinal mucosa were significantly higher in the asthma group( P lt; 0. 05) . There were no significant differences of other indices between the two groups. In the normal group, the eotaxin protein expression had a negative correlation
between lung tissue and rhinal mucosa( r = - 0. 572, P = 0. 008) , and a positive correlation between intestinal and rhinal mucosa( r=0. 638, P =0. 002) . The eotaxin mRNA expression had a positive correlation between lung tissue and rhinal mucosa( r= 0. 502, P = 0. 024) , and a positive correlation between intestinal
and rhinal mucosa( r=0. 594, P =0. 006) . In the asthma group, such a correlation was not found except the eotaxin protein expression which had a negative correlation between lung tissue and intestinal mucosa( r =- 0. 448, P = 0. 048) . Conclusions Mucosal immunity in lung, nose and intestine remains a dynamic balance. The balance of mucosal immunity is destroyed in asthma.

Citation： LI Fengsen,QINHuijuan,HAMULATI. Experimental Study on the Relation of Mucosal Immunity in Asthma Rats’Lung,Nose and Intestine. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(3): 273-275. doi: Copy

1.	中华医学会呼吸病学分会哮喘学组. 支气管哮喘防治指南( 支气管哮喘的定义、诊断、治疗及教育和管理方案) . 中华内科学杂志, 2003 , 42: 817 -822.
2.	Umetsu DT, DeKruyff RH. The regulation of allergy and asthma.Immunol Rev, 2006 , 212: 238-255 .
3.	Wilson JW, Kotsimbos T. Airway vascular remodeling in asthma.Curr Allergy Asthma Rep, 2003, 3: 153 -158.
4.	崔龙苹, 杨永清, 陈汉平, 等. 过敏性哮喘大鼠模型的制备. 上海实验动物科学, 2000, 20: 69 -71 .
5.	Deng YM, Xie QM, Chen JQ, et al. Coincidential increase of leukotriene B4 between cerebral cortex and lung tissue of seneitized rats. Acta Pharmacol Sin, 2003 , 24 : 1039-1044.
6.	Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma( ARIA) 2008 update ( in collaboration with the World Health Organization, GA( 2 ) LEN and AllerGen) . Allergy,2008, 63 Suppl 86: 8-160 .
7.	高杰英. 黏膜免疫向免疫学提出了新问题. 上海免疫学杂志,2000, 20: 257-259 .
8.	Mayer L. Mucosal immunity. Pediatrics, 2003, 111: 1595-1600.
9.	Hideki I, Ichiro T, Hiroshi K. Mucosal immune network in the gut for the control of infectious diseases. Rev Med Virol, 2001, 11: 117 -133.
10.	Makala LHC, Suzuki N, Nagasawa H. Peyer’s patches: organized lymphoid structures for the induction of mucosal immune responsesin the intestine. Pathobiology, 2002, 70 : 55 -68.

1. 中华医学会呼吸病学分会哮喘学组. 支气管哮喘防治指南( 支气管哮喘的定义、诊断、治疗及教育和管理方案) . 中华内科学杂志, 2003 , 42: 817 -822.
2. Umetsu DT, DeKruyff RH. The regulation of allergy and asthma.Immunol Rev, 2006 , 212: 238-255 .
3. Wilson JW, Kotsimbos T. Airway vascular remodeling in asthma.Curr Allergy Asthma Rep, 2003, 3: 153 -158.
4. 崔龙苹, 杨永清, 陈汉平, 等. 过敏性哮喘大鼠模型的制备. 上海实验动物科学, 2000, 20: 69 -71 .
5. Deng YM, Xie QM, Chen JQ, et al. Coincidential increase of leukotriene B4 between cerebral cortex and lung tissue of seneitized rats. Acta Pharmacol Sin, 2003 , 24 : 1039-1044.
6. Bousquet J, Khaltaev N, Cruz AA, et al. Allergic Rhinitis and its Impact on Asthma( ARIA) 2008 update ( in collaboration with the World Health Organization, GA( 2 ) LEN and AllerGen) . Allergy,2008, 63 Suppl 86: 8-160 .
7. 高杰英. 黏膜免疫向免疫学提出了新问题. 上海免疫学杂志,2000, 20: 257-259 .
8. Mayer L. Mucosal immunity. Pediatrics, 2003, 111: 1595-1600.
9. Hideki I, Ichiro T, Hiroshi K. Mucosal immune network in the gut for the control of infectious diseases. Rev Med Virol, 2001, 11: 117 -133.
10. Makala LHC, Suzuki N, Nagasawa H. Peyer’s patches: organized lymphoid structures for the induction of mucosal immune responsesin the intestine. Pathobiology, 2002, 70 : 55 -68.

Chinese Journal of Respiratory and Critical Care Medicine

Experimental Study on the Relation of Mucosal Immunity in Asthma Rats’Lung,Nose and Intestine

Abstract Full text Figures/Tables Video References Cited by

Format

Content