Effects of Heme Oxygenase-1 Intervention on Ventilator-Induced Lung Injury_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ANLi , LIU Qinghui , QIN Xuebing , LIU Changting , YU Senyang

Department of Respiratory Medicine, General Hospital of PLA.Beijing, 100853, ChinaCorresponding Author: YUSen-yang, E-mail: senyangyu@ sina. com;

Corresponding author：

Keywords：

Ventilator-induced lung injury; Heme oxygenase-1; Rabbit

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Objective To explore the expression and effect of heme oxygenase-1 ( HO-1) in ventilator-induced lung injury. Methods Twenty-four New Zealand rabbits were randomly assigned to three groups, ie. a conventional ventilation + PEEP group( C group) , a ventilator-induced lung injury group( VILI group) , and a VILI + HO-1 inducer hemin group( Hm group) .Western blot and immunohistochemistry assay were used to investigate the expression of HO-1 protein. Blood gas analysis, lung wet /dry ratio, lung
histopathology and lung injury score were used to evaluate lung injury. Results HO-1 protein expression significantly increased in the VILI group compared with the C group. HO-1 was found mainly in alveolar epithelial cells and vascular endothelial cells, as well as in alveolar macrophages and neutrophils. Compared with the VILI group, HO-1 protein and PaO2 /FiO2 increased, while lung wet/dry ratio and lung injury score decreased in the Hmgroup significantly( P lt;0. 05) . Conclusion High HO-1 expression can alleviate lung injury from large tidal volume ventilation, implying its protective role in lung pathogenesis.

Citation： ANLi,LIU Qinghui,QIN Xuebing,LIU Changting,YU Senyang. Effects of Heme Oxygenase-1 Intervention on Ventilator-Induced Lung Injury. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(3): 284-286. doi: Copy

1.	薛挥,郭花, 焦捷英. 血红素氧合酶-1 对肝硬化大鼠肝脏缺血再灌注损伤的保护作用. 西安交通大学学报( 医学版) ,2007, 28:47-50.
2.	Song R, Kubo M, Morse D. Carbon monoxide induces cytoprotection in rat orthotopic lung transplantation via anti-inflammatory and antiapoptotic effects. Am J Pathol, 2003, 163: 231 -242.
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5.	秦雪冰, 俞森洋. 呼吸机所致肺损伤家兔HSP70 的表达和细胞因子的关系. 中国现代医学杂志, 2007, 17: 44-47.
6.	Morse D, Choi AM. Heme oxygenase-1: from bench to bedside. Am J Respir Crit Care Med, 2005 , 172: 660-670.
7.	Shan Y, Pepe J, Lambrecht RW, et al. Mapping of the chick heme oxygenase-1 proximal promoter for responsiveness to metalloporphyrins. Arch Biochem Biophys, 2002 , 399: 159-166 .

1. 薛挥,郭花, 焦捷英. 血红素氧合酶-1 对肝硬化大鼠肝脏缺血再灌注损伤的保护作用. 西安交通大学学报( 医学版) ,2007, 28:47-50.
2. Song R, Kubo M, Morse D. Carbon monoxide induces cytoprotection in rat orthotopic lung transplantation via anti-inflammatory and antiapoptotic effects. Am J Pathol, 2003, 163: 231 -242.
3. Sinclair SE, Altemeier WA, Matute-Bello G, et al. Augmented lung injury due to interaction between hyperoxia and mechanical ventilation. Crit Care Med, 2004, 32 : 2496 -2501 .
4. 刘庆辉, 尹建敏, 刘岩, 等. 高浓度氧加重大鼠通气机所致肺损伤的实验研究. 中国呼吸与危重监护杂志, 2007, 6: 197 -200.
5. 秦雪冰, 俞森洋. 呼吸机所致肺损伤家兔HSP70 的表达和细胞因子的关系. 中国现代医学杂志, 2007, 17: 44-47.
6. Morse D, Choi AM. Heme oxygenase-1: from bench to bedside. Am J Respir Crit Care Med, 2005 , 172: 660-670.
7. Shan Y, Pepe J, Lambrecht RW, et al. Mapping of the chick heme oxygenase-1 proximal promoter for responsiveness to metalloporphyrins. Arch Biochem Biophys, 2002 , 399: 159-166 .

Chinese Journal of Respiratory and Critical Care Medicine

Effects of Heme Oxygenase-1 Intervention on Ventilator-Induced Lung Injury

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