Eotaxin Expression of Airway Smooth Muscle Cells Induced by Sensitized Serum in Rats_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

GAO Feng ¹ , XIAO Lei. ²

Department of Respiratory Medicine, Affiliated Hospital of Xuzhou Medical College.Xuzhou, Jiangsu,221002, ChinaCorresponding Author: XIAO Lei, E-mail: pponline@ 163. com;

Corresponding author：

Keywords：

Asthma; Airway smooth muscle cells; Eotaxin; Cyclic adenosine monophosphate

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Objective To observe the eotaxin expression of rat airway smooth muscle cells ( ASMCs) induced by serum from asthmatic rats, and explore the possible mechanism. Methods ASMCs isolated fromrat tracheas were cultured in vivo. Then they were treated with serum from asthmatic rats, or treated with serum and dexamethasone simultaneously. The level of eotaxin protein in supernatant and eotaxin mRNA in ASMCs were measured by ELISA and reverse transcription-polymerase chain reaction. The expression of cAMP in ASMCs was examined by radioimmunoassay. Results After the treatment with sensitized serum, the eotaxin level in supernatant and mRNA expression in ASMCs were significantly higher [ ( 107. 09 ±7. 12) ng/L vs. ( 0. 63 ±0. 56) ng/L, P lt; 0. 05; 1. 39 ±0. 04 vs. 0. 05 ±0. 01, P lt;0. 05] , and the level of cAMP in ASMCs was significantly lower compared with the control group [ ( 17. 58 ±3. 62) ng/L vs. ( 32. 39 ±3. 36) ng/L, P lt; 0. 05] . After intervened by the sensitized serum and dexamethasone simultaneously, the protein and mRNA expressions of eotaxin were lower compared with those intervened by sensitized serumalone [ ( 64. 18 ±4. 04) ng/L and 0. 77 ±0. 19] . The level of eotaxin in supernatant was negatively correlated with cAMP level in ASMCs ( r = - 0. 788, P lt; 0. 01) . Conclusions There is an
autocrine function in ASMCs as inflammatory cells after stimulation with sensitized serum. Eotaxin may play an important roll in the pathogenesis of asthma via a cAMP-dependent pathway.

Citation： GAO Feng,XIAO Lei.. Eotaxin Expression of Airway Smooth Muscle Cells Induced by Sensitized Serum in Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(5): 490-492. doi: Copy

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8.	Hakonarson H, Grunstein MM. Autocrine regulation of airway smooth muscle responsiveness. Respir Physiol Neurobiol, 2003,137: 263-276.
9.	Rahman MS, Yamasaki A, Yang J, et al. I L-17A induces eotaxin-1 /CC chemokine ligand 11 expression in human airway smooth muscle cells: role of MAPK ( Erk1 /2, JNK, and p38) pathways. J Immunol,2006, 177: 4064-4071.
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11.	张丽娜, 陈萍. 支气管哮喘靶向治疗的新进展. 中国呼吸与危重监护杂志, 2009, 8 : 411-414.
12.	Tliba O, Panettieri RA Jr. Noncontractile function of airway smooth muscle cells in asthma. Annu Rev Physiol, 2009, 71: 509 -535.

1. Peng T, Hao L, Madri JA, et al. Role of C5 in the development of airway inflammation, airway hyperresponsiveness, and ongoing airway response. J Clin Invest, 2005 , 115: 1590-1600.
2. Panettieri RA Jr. Airway smooth muscle: immunomodulatory cells that modulate airway remodeling. Respir Physiol Neurobiol, 2003 ,137: 277-293.
3. Xu SY, Xu YJ, Zhang ZX, et al. Contribution of protein kinase C to passively sensitized human airway smooth muscle cells proliferation.Chin Med J( Engl) , 2004, 117: 30 -36.
4. Weltman JK. Cytokines: regulators of eosinophilic inflammation.Allergy Asthma Proc, 2000, 21: 203-207 .
5. Lilly CM, Nakamura H, Kesselman H, et al. Expression of eotaxin by human lung epithelial cells: induction by cytokines and inhibition by glucocorticoids. J Clin Invest, 1997, 99 : 1767 -1773 .
6. Zuyderduyn S, Sukkar MB, Fust A, et al. Treating asthma means treating airway smooth muscle cells. Eur Respir J,2008, 32: 265-274.
7. Tliba O, Panettieri RA Jr. Regulation of Inflammation by airway smooth muscle. Curr Allergy Asthma Rep, 2008, 8: 262-268.
8. Hakonarson H, Grunstein MM. Autocrine regulation of airway smooth muscle responsiveness. Respir Physiol Neurobiol, 2003,137: 263-276.
9. Rahman MS, Yamasaki A, Yang J, et al. I L-17A induces eotaxin-1 /CC chemokine ligand 11 expression in human airway smooth muscle cells: role of MAPK ( Erk1 /2, JNK, and p38) pathways. J Immunol,2006, 177: 4064-4071.
10. Zuyderduyn S, Sukkar MB, Fust A, et al. Treating asthma means treating airway smooth muscle cells. Eur Respir J,2008, 32: 265-274.
11. 张丽娜, 陈萍. 支气管哮喘靶向治疗的新进展. 中国呼吸与危重监护杂志, 2009, 8 : 411-414.
12. Tliba O, Panettieri RA Jr. Noncontractile function of airway smooth muscle cells in asthma. Annu Rev Physiol, 2009, 71: 509 -535.

Chinese Journal of Respiratory and Critical Care Medicine

Eotaxin Expression of Airway Smooth Muscle Cells Induced by Sensitized Serum in Rats

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