The Roles of CC-16, Transcription Factor T-bet, and GATA-3 in Airway Inflammation of Patients with Asthma_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

DU Xiufang , HUANG Ping , LI Yilu , LI Zhiying

Department of Respiratory Disease,Affiliated Nanshan Hospital, Guangdong Medical College. Shenzhen, Guangdong, 518052, ChinaCorresponding Author: DU Xiu-fang, E-mail: duxiufang321@ 163. com;

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Keywords：

Asthma; Clara cell secretory 16 kD protein; T-bet; GATA-3; Airway inflammation

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Objective To investigate the modulating roles of Clara cell secretory 16 kD protein ( CC-16) , transcription factor T-bet, and GATA-3 in airway inflammation of patients with asthma. Methods 25 patients with acute exacerbation of asthma were enrolled as an asthma group and 33 healthy volunteers were enrolled as control. The plasma levels of CC16, IFN-γ, and IL-4 were measured by enzyme-linked immunosorbent assay ( ELISA) . The mRNA expressions of T-bet and GATA-3 in the peripheral blood
mononuclear cells ( PBMCs) were measured by reverse transcription-polymerase chain reaction ( RT-PCR) .Results The levels of CC16 and IFN-γin the asthma group were lower than those in the control group [ ( 21. 96 ±7. 31 ) ng/mL vs. ( 64. 88 ±25. 27) ng/mL, ( 118. 73 ±22. 59) pg/mL vs. ( 145. 53 ±29. 50) pg/mL, both P lt;0. 01] . The IL-4 level in the asthma group was significantly higher than that in the control group [ ( 425. 22 ±4. 37) pg/mL vs. ( 69. 72 ±10. 15 ) pg/mL, P lt; 0. 01] . The T-bet mRNA expression and T-bet /GATA-3 ratio of PBMCs in the asthma group were significantly lower than those in the control group( both P lt; 0. 01) . The expression GATA-3 mRNA was significantly higher than that in the control group( P lt;0. 01) . The level of CC16 was positively correlated with T-bet mRNA expression and the ratio of T-bet /GATA-3 ( r =0. 792, 0. 761, respectively, P lt; 0. 01) . There was no correlation between CC16 and the GATA-3 mRNA expression ( P gt;0. 05) . Conclusions These results suggest that CC16 and T-bet play important protection roles in the pathogenesis of asthma. GATA-3, IFN-γ, and IL-4 also participate in the airway inflammation of asthma.

Citation： DU Xiufang,HUANG Ping,LI Yilu,LI Zhiying. The Roles of CC-16, Transcription Factor T-bet, and GATA-3 in Airway Inflammation of Patients with Asthma. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(6): 611-613. doi: Copy

1.	潘世秀, 曾天舒, 崔天盆, 等, 吡格列酮对哮喘模型Th1 / Th2 细胞因子表达的影响, 中华微生物学和免疫学杂志, 2006, 3: 237 -240.
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1. 潘世秀, 曾天舒, 崔天盆, 等, 吡格列酮对哮喘模型Th1 / Th2 细胞因子表达的影响, 中华微生物学和免疫学杂志, 2006, 3: 237 -240.
2. Hung CH, Chen LC, Zhang Z, et al. Regulation of Th2 responses by the pulmonary Clara cell secretory 10 -kd protein. J Allergy Clin Immunol, 2004, 114: 664 -670.
3. Pai SY, Truitt ML, Ho IC. GATA-3 deficiency abrogates the developmentand maintenance of T helper type 2 cells. Proc Natl Acad Sci USA, 2004, 101: 1993-1998.
4. de Burbure C, Pignatti P, Corradi M, et al. Uteroglobin-Related protein and clara cell protein in inducedsputum of patients with asthma and rhinitis. Chest, 2007, 131 : 172-179.
5. Johansson S, Wennergren G, Aberg N, et al. Clara cell 16-kd protein downregulates T ( H) 2 differentiation of human naive neonatal T cells. J Allergy Clin Immunol, 2007, 120 : 308-314.
6. Nakamura Y, Hoshino M. TH2 cytokines and associated transcription factors as therapeutic targets in asthma. Curr Drug Targets Inflamm Allergy, 2005, 4: 267 -270.
7. Justice JP, Borchers MT, Lee JJ, et al. Ragweed- induced expression of. GATA-3, IL-4, and IL-5 by eosinophils in the lungs of allergic C57BL/6J mice. Am J Physiol Lung Cell Mol Physiol, 2002 , 282 :L302-L309.
8. Inman D, Kawana K, Schust D, et al. Cyclic regulation of T-Bet and GATA-3 in human endometrium. Reprod Sci, 2008, 15: 83 -90 .

Chinese Journal of Respiratory and Critical Care Medicine

The Roles of CC-16, Transcription Factor T-bet, and GATA-3 in Airway Inflammation of Patients with Asthma

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