GU Wen , GUO Xuejun , DING Tao
  • Department of Respiratory Medicine, Xinhua Hospital,Medical School of Shanghai Jiaotong University. Shanghai, 200092, China Corresponding Author: GUO Xue-jun, E-mail: guoxjz@ yahoo. com. cn;
Asthma; β1,3-N-acetylglucosaminyltransferases; RNA interfering; CD4 + T lymphocyte
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Objective  To investigate the effects of down-regulating of Rfng gene ( 1, 3-Nacetylglucosaminyltransferases) in lung CD4 + T cells of asthmatic rat model by small interfering RNA ( siRNA) and explore the role of Rfng in pathogenesis of asthma.
Methods  An asthmatic rat model was established by OVA sensitization and challenge. Total T cells were isolated from lung tissue of asthmatic rats, and CD4 + T lymphocytes were purified using magnetic beads. CD4 + T lymphocytes were transfected by siRNA targeting Rfng gene. The mRNA and protein expressions of Rfng were detected by quantitative PCR and Western blot. Quantitative PCR was performed to determine the mRNA levels of Th1 /Th2 cytokines and related genes including IL-12, IFN-γ, IL-4, IL-5, T-bet, and GATA3. ELISA was performed to determine the concentrations of IL-12, IFN-γ, IL-4, and IL-5 in supernatant.
Results  The mRNA and protein expression of Rfng in RNAi group decreased significantly. IL-12, IFN-γ, T-bet increased and while IL-4, IL-5, and GATA3 decreased significantly. The concentrations of IL-12 and IFN-γ in the supernatant increased significantly, while IL-4 and IL-5 decreased significantly.
Conclusions  Down regulation of Rfng affects T cell differentiation. It is presumed that Fringe contribute to the pathogenesis of asthma.

Citation： GU Wen,GUO Xuejun,DING Tao. Small Interfering RNA-Mediated Knockdown of Fringe on Extracellular Domain of Notch Affects T Cell Differentiation. Chinese Journal of Respiratory and Critical Care Medicine, 2011, 10(4): 325-330. doi: Copy