HAN Zhaoqing , QIAO Jianou , WANG Jian
  • Shanghai Ninth People’s Hospital, Shanghai Jiaotong University. Shanghai, 210011,ChinaCorresponding Author: WANG Jian, E-mail: wangjian2013@ yahoo. com. cn;
Diesel exhaust particle; Eotaxin; Asthma
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective  To investigate the effects of diesel exhaust particles ( DEP) on the production of CCL11, CCL24 and CCL26 in asthmatic rats.
Methods  Fifty SD rats were randomly divided into five groups. Group A was an normal control group. The rats in group B, C, D, and E were sensitized and challenged by ovalbumin ( OVA) to establish asthma model. Then the rats in the group C, D, E were inhaled DEP for 1, 2, 3 weeks, respectively. Lung tissue and brouchoalveolar lavage fluid ( BALF) were collected for detection of CCL11, CCL24, and CCL26 expression by ELISA and q-RT-PCR.
Results  The transcription of CCL 24, CCL26 gene and the production of CCL24 and CCL26 protein increased significantly compared with the control group ( P  lt;0. 05) , and were positively associated with the DEP inhalation time. However, CCL11 gene and protein expression were not changed significantly compared with the control.
Conclusion  The exposure to DEP can induce the production of CCL24 and CCL26 in the asthmaic rats, which might aggravate
airway hyperresponsiveness.

Citation： HAN Zhaoqing,QIAO Jianou,WANG Jian. Effects of Diesel Exhaust Particle on Expression of Eotaxin in Asthmatic Rats. Chinese Journal of Respiratory and Critical Care Medicine, 2011, 10(5): 451-453. doi: Copy