• Department of Respiratory Medicine, Nanjing Hospital Affiliated to Nanjing Medical University ( Nanjing First Hospital) . Nanjing, Jiangsu,210006, China;
Chronic obstructive pulmonary disease; Pulmonary surfactant protein D; Lipopolysaccharide
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Objective  To investigate the relationship of pulmonary surfactant protein D( SP-D) with chronic obstructive pulmonary disease ( COPD) by measuring SP-D level in serum and lung tissue of rats with COPD.
Methods  The rat COPD model was established by passive smoking as well as intratracheal instillation of lipopolysaccharide ( LPS) . Thirty male SD rats were randomly divided into a control group, a LPS group, and a COPD group( n =10 in each group) . The pathologic changes of lung tissue and airway were observed under light microscope by HE staining. Emphysema changes were evaluated by mean linear intercept ( MLI) of lung and mean alveolar number ( MAN) . The level of SP-D in serum was measured by enzymelinked immunosorbent assay ( ELISA) . The expression of SP-D in lung tissue was detected by Western-blot and immunohistochemistry.
Results  The MLI obviously increased, and MAN obviously decreased in the COPD group compared with the control group ( P lt;0.05) . There was no significant difference in the MLI and MAN between the LPS group and the control group ( P gt;0.05) . The serum SP-D level was ( 49.59 ±2.81) ng/mL and ( 53.21±4.17) ng/mL in the LPS group and the COPD group, which was significantly higher than that in the control group [ ( 42.14±2.52) ng/mL] ( P lt;0.05) . The expression of SP-D in lung tissue was 0.56±0.01 and 0.63±0.01 in the LPS group and the COPD group, which was also obviously ber than that in the control group ( 0.39 ±0.01) ( P lt;0.05) .Meanwhile the SP-D levels in serumand lung tissue were higher in the COPD group than those in the LPS group ( P lt;0.05) . The levels of SP-D between serum and lung tissue were positively correlated in all three groups ( r=0.93, 0.94 and 0.93, respectively, P lt;0.01) .
Conclusion  Both the SP-D level in serum and in lung tissue increase significantly in COPD rats and correlate well each other, which suggests that SP-D may serve as a biomarker of COPD.

Citation： ZHANG Jinmei,SUN Lihua,GU Wei,TAN Yan,FANG Surong. Changes of Pulmonary Surfactant Protein D in Serum and Lung Tissue of Rats with Chronic Obstructive Pulmonary Disease. Chinese Journal of Respiratory and Critical Care Medicine, 2012, 11(4): 330-334. doi: Copy