LIU Kaiyu , TIAN Hai , LIU Wei , et al .
  • 1. Department of Cardiovascular Surgery, the Second Affiliated Hospital, Harbin Medical University, Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment, Harbin 150086, P. R. China;;
  • 2. Department of Cardiovascular Surgery, Toronto General Hospital, University of Toronto, M5G2C4 Canada;
Smooth muscle cell transplantation; Myocardial remodeling; Tissue inhibitor of metalloprotease-3; Matrix metalloproteinase-2; Matrix metalloproteinase-9
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Objective To evaluate the effect of smooth muscle cell transplantation on myocardial interstitial reconstruction shortly after myocardial infarction. Methods A total of 48 female Wister rats were randomly divided into two groups with the random number table, the control group (n=24) and the smooth muscle cell transplantation group (n=24). The left coronary artery was ligated to set up the myocardial infarction animal model. An amount of 05 ml phosphate buffered saline(PBS) containing 1×106 smooth muscle cells or 0.5 ml PBS without cells was injected into the injured myocardium immediately. By immunoblot and reverse transcriptionolymerase china reaction (RT-PCR), we observed the amount of protein and mRNA of matrix metalloproteinase2(MMP-2), matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloprotease-3 (TIMP-3) in the myocardium of the rats. Results The transplanted smooth muscle cells survived well. Compared with the control group, myocardial TIMP3 mRNA (1.06±0.22 vs. 0.81±0.19, t=－2.358, P=0.033) and protein content (3.33±0.53 vs. 1.63±0.47, t=－6.802, P lt;0.001) were significantly increased in the transplantation group. Myocardial MMP-2, MMP-9 mRNA (0.49±0.12 vs. 1.16±0.18, t=8.453, P lt;0.001; 0.45±0.12 vs. 0.80±0.11, t=5.884, P lt;0.001) and protein content (3.98±1.08 vs. 6.05±0.91, t=4.139, P=0.001; 0.39±0.14 vs. 0.57±0.17, t=2.409, P=0.031) [CM(1585mm]were significantly reduced in the transplantation group compared with the control group. Conclusion transplanted smooth muscle cells can survive well in the infarction myocardium and can increase the amount of myocardial TIMP-3 mRNA and protein content and reduce myocardial MMP-2, MMP-9 mRNA and protein content, which is an effective way to prevent harmful cardiac remodeling.

Citation： LIU Kaiyu,TIAN Hai,LIU Wei,et al .. The Effect of Smooth Muscle Cell Transplantation on MyocardialMMP2, MMP9 and TIMP3 Content One Week after Myocardial Infarction. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2010, 17(3): 219-224. doi: Copy