XIONG Jian , ZHONG Hong , CHEN Sufeng , et al .
  • Department of Thoracic and Cardiovascular Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, P.R. China;
Endothelial progenitor cells; Recombinant adenovirus; LacZ gene; Multiplicity of infection
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Objective To determine the transfection efficiency of recombinant adenovirus to endothelial progenitor cells(EPCs) and provide the base of lung cancer therapy by transfecting human herpes simplex virusthymidine kinase（HSV-TK） gene to EPCs. Methods Admove recombinant adenovirus 5F35(AD5F35) which transfected with βgalactosidase(AD5F35LacZ) to the 24 well plate cultivated with EPCs and transfect the EPCs. Stain the EPCs with LacZ kit and calculate the transfection efficiency. Results The blue stain cells were cells transfected successfully with AD5F35LacZ under the optical microscope. The transfection efficiencies of adenovirus to EPCs were different under the premise of the different multiplicity of infection(MOI). In a certain range, the transfection efficiencies rise with the MOI rise. When MOI was 400,the proportion of blue stain cell is the highest, which was 98.38%±1.25%. Conclusion Recombinant adenovirus can transfect EPCs successfully. The transfection efficiencies rise with the MOI rise. When the MOI is 400,the transfection efficiency is the highest.

Citation： XIONG Jian,ZHONG Hong,CHEN Sufeng,et al .. The Experimental Studies of Recombinant Adenovirus Vectormediated βGalactosidase Gene Transfected to Endothelial Progenitor Cells. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2009, 16(4): 283-286. doi: Copy