SUN Hongtao , WU Qingyu , XU Jianping.
  • Department of Cardiovascular Surgery, Fu Wai Hospital and Cardiovascular Institute, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100037, P. R. China.;
c-myc; Proliferating cell nuclear antigen; Soluble stent; Antisense oligodeoxynucleotides
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Objective To investigate the effect on expression of c-myc and proliferating cell nuclear antigen （PCNA） of vein grafts transferred by c-myc antisense oligodeoxynucleotides（ODN） of soluble stent. Methods A rabbit model of common carotid arteries grafted by external jugular veins was constructed in 50 New Zealand rabbits and were randomly divided into five groups, 10 rabbits each group. Control group： no stents ; group 1： soluble stent ; group 2： soluble stent with sense-ODN; group 3： soluble stent with antisense-ODN; group 4.. soluble stent with mismatch-ODN. At 7 d, 28 d and 90 d after surgery, vein grafts were harvested. The expression of c-myc and PCNA were identified by immunochemistry methods. Results At 7d, 28d, 90d after surgery, the expression of c-myc and PCNA of the intima and media of vein grafts in control group, group 1, group 2, group 4 were higher significantly than that in group 3 （P〈0. 01）. At 28d, 90d after surgery, the expression of c-myc in five groups were higher than that in the same group at 7d after surgery （P〈0. 01）. Conclusion Soluble stent can transfer ODN effectively. C- myc antisense-ODN transferred by soluble stent can inhibit significantly the expression of c-myc and PCNA in the intima and media of vein grafts.

Citation： SUN Hongtao,WU Qingyu,XU Jianping.. Effect on the Expression of C-myc and Proliferating Cell Nuclear Antigen of Vein Grafts Transferred by C-myc Antisense Oligodeoxynucleotides of Soluble Stent. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2006, 13(3): 176-179. doi: Copy