RELATED ISSUES IN CLINICAL TRANSLATIONAL APPLICATION OF ADIPOSE-DERIVED STEM CELLS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LIU Hongwei ¹ , CHENG Biao ² , FU Xiaobing ³

1Department of Plastic Surgery, the First Affiliated Hospital of Jinan University, Key Laboratory of Regenerative Medicine, Ministry of Education, Guangzhou Guangdong, 510630, P.R.China....详见正文;

Corresponding author：

Keywords：

Adipose-derived stem cells; Stromal vascular fraction cells; Translation medicine; Regenerative medicine

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Objective To introduce the related issues in the clinical translational application of adipose-derived stem cells (ASCs). Methods The latest papers were extensively reviewed, concerning the issues of ASCs production, management, transportation, use, and safety during clinical application. Results ASCs, as a new member of adult stem cells family, bring to wide application prospect in the field of regenerative medicine. Over 40 clinical trials using ASCs conducted in 15 countries have been registered on the website (http://www.clinicaltrials.gov) of the National Institutes of Health (NIH), suggesting that ASCs represents a promising approach to future cell-based therapies. In the clinical translational application, the related issues included the quality control standard that management and production should follow, the prevention measures of pathogenic microorganism pollution, the requirements of enzymes and related reagent in separation process, possible effect of donor site, age, and sex in sampling, low temperature storage, product transportation, and safety. Conclusion ASCs have the advantage of clinical translational application, much attention should be paid to these issues in clinical application to accelerate the clinical translation process.

Citation： LIU Hongwei,CHENG Biao,FU Xiaobing. RELATED ISSUES IN CLINICAL TRANSLATIONAL APPLICATION OF ADIPOSE-DERIVED STEM CELLS. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(10): 1242-1246. doi: Copy

1.	Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
2.	Gimble JM, Bunnell BA, Guilak F. Human adipose-derived cells: an update on the transition to clinical translation. Regen Med, 2012, 7(2): 225-235.
3.	Gimble JM, Bunnell BA, Chiu ES, et al. Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation. Stem Cells, 2011, 29(5): 749-754.
4.	Sensebé L, Bourin P, Tarte K. Good manufacturing practices production of mesenchymal stem/stromal cells. Hum Gene Ther, 2011, 22(1): 19-26.
5.	Lin K, Matsubara Y, Masuda Y, et al. Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy, 2008, 10(4): 417-426.
6.	Güven S, Karagianni M, Schwalbe M, et al. Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax® technology. Tissue Eng Part C Methods, 2012. [Epub ahead of print].
7.	黄海玲, 刘宏伟, 佘文莉, 等. 介绍一种微创无菌快速获取可移植脂肪颗粒装置. 中华整形外科杂志, 2011, 27(6): 467-468.
8.	Doi K, Tanaka S, Iida H, et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med, 2012. [Epub ahead of print].
9.	Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portion of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
10.	Lindroos B, Aho KL, Kuokkanen H, et al. Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum. Tissue Eng Part A, 2010, 16(7): 2281-2294.
11.	王太平, 徐国彤, 周琪, 等. 国际干细胞研究学会《干细胞临床转化指南》. 生命科学, 2009, 21(5): 747-756.
12.	Fink T, Rasmussen JG, Lund P, et al. Isolation and expansion of adipose-derived stem cells for tissue engineering. Front Biosci (Elite Ed), 2011, 3: 256-263.
13.	van Harmelen V, Skurk T, Röhrig K, et al. Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int J Obes Relat Metab Disord, 2003, 27(8): 889-895.
14.	Mojallal A, Lequeux C, Shipkov C, et al. Influence of age and body mass index on the yield and proliferation capacity of adipose-derived stem cells. Aesthetic Plast Surg, 2011, 35(6): 1097-1105.
15.	Ogawa R, Mizuno H, Watanabe A, et al. Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice-including relationship of sex differences. Biochem Biophys Res Commun, 2004, 319(2): 511-517.
16.	Aksu AE, Rubin JP, Dudas JR, et al. Role of gender and anatomical region on induction of osteogenic differentiation of human adipose-derived stem cells. Ann Plast Surg, 2008, 60(3): 306-322.
17.	Chen HT, Lee MJ, Chen CH, et al. Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures. J Cell Mol Med, 2012, 16(3): 582-593.
18.	Shu W, Shu YT, Yundai C, et al. Comparing the biological characteristics of adipose tissue-derived stem cells of different persons. J Cell Biochem, 2012, 113(6): 2020-2026.
19.	Alt EU, Senst C, Murthy SN, et al. Aging alters tissue resident mesenchymal stem cell properties. Stem Cell Res, 2012, 8(2): 215-225.
20.	Sowa Y, Imura T, Numajiri T, et al. Adipose-derived stem cells produce factors enhancing peripheral nerve regeneration: influence of age and anatomic site of origin. Stem Cells Dev, 2012, 21(11): 1852-1862.
21.	陈燕, 陆志刚, 白海, 等. 细胞内外海藻糖对红细胞冰冻干燥保存的影响. 中国输血杂志, 2012, 25(3): 236-239.
22.	刘广鹏, 李宇琳, 孙剑, 等. 低温冻存对人脂肪来源干细胞成骨能力影响的实验研究. 中国修复重建外科杂志, 2010, 24(10): 1224-1227.
23.	Feng Z, Ting J, Alfonso Z, et al. Fresh and cryopreserved, uncultured adipose tissue-derived stem and regenerative cells ameliorate ischemia-reperfusion-induced acute kidney injury. Nephrol Dial Transplant, 2010, 25(12): 3874-3884.
24.	Lee JE, Kim I, Kim M. Adipogenic differentiation of human adipose tissue-derived stem cells obtained from cryopreserved adipose aspirates. Dermatol Surg, 2010, 36(7): 1078-1083.
25.	Martinello T, Bronzini I, Maccatrozzo L, et al. Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation. Res Vet Sci, 2011, 91(1): 18-24.
26.	James AW, Levi B, Nelson ER, et al. Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo. Stem Cells Dev, 2011, 20(3): 427-439.
27.	Matsumoto D, Shigeura T, Sato K, et al. Influences of preservation at various temperatures on liposuction aspirates. Plast Reconstr Surg, 2007, 120(6): 1510-1517.
28.	Prockop DJ, Brenner M, Fibbe WE, et al. Defining the risks of mesenchymal stromal cell therapy. Cytotherapy, 2010, 12(5): 576-578.
29.	Ra JC, Kang SK, Shin IS, et al. Stem cell treatment for patients with autoimmune disease by systemic infusion of culture-expanded autologous adipose tissue derived mesenchymal stem cells. J Transl Med, 2011, 9: 181.
30.	López-Iglesias P, Blázquez-Martínez A, Fernández-Delgado J, et al. Short and long term fate of human AMSC subcutaneously injected in mice. World J Stem Cells, 2011, 3(6): 53-62.
31.	Sun B, Roh KH, Park JR, et al. Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model. Cytotherapy, 2009, 11(3): 289-298.
32.	Pinilla S, Alt E, Abdul Khalek FJ, et al. Tissue resident stem cells produce CCL5 under the influence of cancer cells and thereby promote breast cancer cell invasion. Cancer Lett, 2009, 284(1): 80-85.
33.	Zimmerlin L, Donnenberg AD, Rubin JP, et al. Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates. Tissue Eng Part A, 2011, 17(1-2): 93-106.
34.	Muehlberg FL, Song YH, Krohn A, et al. Tissue-resident stem cells promote breast cancer growth and metastasis. Carcinogenesis, 2009, 30(4): 589-597.
35.	Donnenberg VS, Zimmerlin L, Rubin JP, et al. Regenerative therapy after cancer: what are the risks? Tissue Eng Part B Rev, 2010, 16(6): 567-575.

1. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
2. Gimble JM, Bunnell BA, Guilak F. Human adipose-derived cells: an update on the transition to clinical translation. Regen Med, 2012, 7(2): 225-235.
3. Gimble JM, Bunnell BA, Chiu ES, et al. Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation. Stem Cells, 2011, 29(5): 749-754.
4. Sensebé L, Bourin P, Tarte K. Good manufacturing practices production of mesenchymal stem/stromal cells. Hum Gene Ther, 2011, 22(1): 19-26.
5. Lin K, Matsubara Y, Masuda Y, et al. Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy, 2008, 10(4): 417-426.
6. Güven S, Karagianni M, Schwalbe M, et al. Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax® technology. Tissue Eng Part C Methods, 2012. [Epub ahead of print].
7. 黄海玲, 刘宏伟, 佘文莉, 等. 介绍一种微创无菌快速获取可移植脂肪颗粒装置. 中华整形外科杂志, 2011, 27(6): 467-468.
8. Doi K, Tanaka S, Iida H, et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med, 2012. [Epub ahead of print].
9. Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portion of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
10. Lindroos B, Aho KL, Kuokkanen H, et al. Differential gene expression in adipose stem cells cultured in allogeneic human serum versus fetal bovine serum. Tissue Eng Part A, 2010, 16(7): 2281-2294.
11. 王太平, 徐国彤, 周琪, 等. 国际干细胞研究学会《干细胞临床转化指南》. 生命科学, 2009, 21(5): 747-756.
12. Fink T, Rasmussen JG, Lund P, et al. Isolation and expansion of adipose-derived stem cells for tissue engineering. Front Biosci (Elite Ed), 2011, 3: 256-263.
13. van Harmelen V, Skurk T, Röhrig K, et al. Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int J Obes Relat Metab Disord, 2003, 27(8): 889-895.
14. Mojallal A, Lequeux C, Shipkov C, et al. Influence of age and body mass index on the yield and proliferation capacity of adipose-derived stem cells. Aesthetic Plast Surg, 2011, 35(6): 1097-1105.
15. Ogawa R, Mizuno H, Watanabe A, et al. Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice-including relationship of sex differences. Biochem Biophys Res Commun, 2004, 319(2): 511-517.
16. Aksu AE, Rubin JP, Dudas JR, et al. Role of gender and anatomical region on induction of osteogenic differentiation of human adipose-derived stem cells. Ann Plast Surg, 2008, 60(3): 306-322.
17. Chen HT, Lee MJ, Chen CH, et al. Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures. J Cell Mol Med, 2012, 16(3): 582-593.
18. Shu W, Shu YT, Yundai C, et al. Comparing the biological characteristics of adipose tissue-derived stem cells of different persons. J Cell Biochem, 2012, 113(6): 2020-2026.
19. Alt EU, Senst C, Murthy SN, et al. Aging alters tissue resident mesenchymal stem cell properties. Stem Cell Res, 2012, 8(2): 215-225.
20. Sowa Y, Imura T, Numajiri T, et al. Adipose-derived stem cells produce factors enhancing peripheral nerve regeneration: influence of age and anatomic site of origin. Stem Cells Dev, 2012, 21(11): 1852-1862.
21. 陈燕, 陆志刚, 白海, 等. 细胞内外海藻糖对红细胞冰冻干燥保存的影响. 中国输血杂志, 2012, 25(3): 236-239.
22. 刘广鹏, 李宇琳, 孙剑, 等. 低温冻存对人脂肪来源干细胞成骨能力影响的实验研究. 中国修复重建外科杂志, 2010, 24(10): 1224-1227.
23. Feng Z, Ting J, Alfonso Z, et al. Fresh and cryopreserved, uncultured adipose tissue-derived stem and regenerative cells ameliorate ischemia-reperfusion-induced acute kidney injury. Nephrol Dial Transplant, 2010, 25(12): 3874-3884.
24. Lee JE, Kim I, Kim M. Adipogenic differentiation of human adipose tissue-derived stem cells obtained from cryopreserved adipose aspirates. Dermatol Surg, 2010, 36(7): 1078-1083.
25. Martinello T, Bronzini I, Maccatrozzo L, et al. Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation. Res Vet Sci, 2011, 91(1): 18-24.
26. James AW, Levi B, Nelson ER, et al. Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo. Stem Cells Dev, 2011, 20(3): 427-439.
27. Matsumoto D, Shigeura T, Sato K, et al. Influences of preservation at various temperatures on liposuction aspirates. Plast Reconstr Surg, 2007, 120(6): 1510-1517.
28. Prockop DJ, Brenner M, Fibbe WE, et al. Defining the risks of mesenchymal stromal cell therapy. Cytotherapy, 2010, 12(5): 576-578.
29. Ra JC, Kang SK, Shin IS, et al. Stem cell treatment for patients with autoimmune disease by systemic infusion of culture-expanded autologous adipose tissue derived mesenchymal stem cells. J Transl Med, 2011, 9: 181.
30. López-Iglesias P, Blázquez-Martínez A, Fernández-Delgado J, et al. Short and long term fate of human AMSC subcutaneously injected in mice. World J Stem Cells, 2011, 3(6): 53-62.
31. Sun B, Roh KH, Park JR, et al. Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model. Cytotherapy, 2009, 11(3): 289-298.
32. Pinilla S, Alt E, Abdul Khalek FJ, et al. Tissue resident stem cells produce CCL5 under the influence of cancer cells and thereby promote breast cancer cell invasion. Cancer Lett, 2009, 284(1): 80-85.
33. Zimmerlin L, Donnenberg AD, Rubin JP, et al. Regenerative therapy and cancer: in vitro and in vivo studies of the interaction between adipose-derived stem cells and breast cancer cells from clinical isolates. Tissue Eng Part A, 2011, 17(1-2): 93-106.
34. Muehlberg FL, Song YH, Krohn A, et al. Tissue-resident stem cells promote breast cancer growth and metastasis. Carcinogenesis, 2009, 30(4): 589-597.
35. Donnenberg VS, Zimmerlin L, Rubin JP, et al. Regenerative therapy after cancer: what are the risks? Tissue Eng Part B Rev, 2010, 16(6): 567-575.

Chinese Journal of Reparative and Reconstructive Surgery

RELATED ISSUES IN CLINICAL TRANSLATIONAL APPLICATION OF ADIPOSE-DERIVED STEM CELLS

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