PROMOTING EFFECT OF MASSAGE ON QUADRICEPS FEMORIS REPAIR OF RABBIT IN VIVO_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

HOU Yixuan ¹ , LIU Manran ² , YU Min ¹ , ZHANG Ping ³ , LIU Zuli ¹ , TANG Chenglin. ¹

1Traditional Chinese Medical College, Chongqing Medical University, Chongqing, 400016, P.R.China;;
2Key Laboratory of Medical Diagnostics of Ministry of Education, Chongqing Medical University;;
3Department of Internal Pediatrics, Enshi Municipal Maternal and Children Care Hospital.;

Corresponding author：

Keywords：

Massage; Skeletal muscle injury repair; Cytoskeletal protein; Rabbit

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【Abstract】 Objective To investigate the effect of massage on quadriceps femoris repair and the expressions of Desmin and α-Actin in rabbits so as to explore the possible molecular mechanisms of massage in repair of muscle injury. Methods Twenty-seven New Zealand white rabbits, weighing (2.0 ± 0.5) kg, were randomly divided into 3 groups: groups A (n=3), B (n=12), and C (n=12). In group A, the rabbits were not treated as controls; in groups B and C, the rabbit models of quadriceps femoris injury were prepared by self-made beater. In group B, no massage therapy was given as nature recovery controls; in group C, RT-N2 intelligent massage device was used for massage therapy at 8 days after injury, at 3 000-3 100 r/min for 15 minutes, every day for 7 days or for 14 days. The quadriceps femoris specimens were taken from 6 rabbits of groups B and C at 14 days and 21 days, respectively. HE staining was employed to detect the histomorphological change. Immunohistochemistry staining and Western blot were used to detect Desmin and α-Actin expressions. The massage therapy effect was evaluated by the histomorphological change and Desmin and α-Actin expressions. Results All rabbits survived to the end of experiment in groups B and C. No histological change was found with regular order of muscle fibers and no connective tissue in group A; obvious tissue necrosis was seen with broken muscular fibers, muscle atrophy, and irregular order in group B; and in group C, the skeletal muscle morphology and muscle atrophy were obviously improved with regenerated muscle fibers when compared with group B. Immunohistochemistry staining showed that the Desmin and α-Actin expressions obviously reduced in groups B and C, which were significantly weaker than that in group A (P lt; 0.05); the Desmin and α-Actin expressions were significantly ber in group C than in group B (P lt; 0.05), and at 21 days than at 14 days in group C (P lt; 0.05). Western blot results showed that the Desmin and α-Actin expressions were significantly higher in group A than in groups B and C (P lt; 0.05), and the expressions were lowest at 14 days in group B. Conclusion The histomorphology and cytoskeletal structure can be significantly improved after massage, which may help to repair muscle injury by up-regulation of Desmin and α-Actin expressions.

Citation： HOU Yixuan,LIU Manran,YU Min,ZHANG Ping,LIU Zuli,TANG Chenglin.. PROMOTING EFFECT OF MASSAGE ON QUADRICEPS FEMORIS REPAIR OF RABBIT IN VIVO. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(3): 346-351. doi: Copy

1.	11 Muroya S, Ertbjerg P, Pomponio L, et al. Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle. Meat Sci, 2010, 86(3): 764-769.
2.	Zainuddin Z, Newton M, Sacco P, et al. Effects of massage on delayed-onset muscle soreness, swelling, and recovery of muscle function. J Athl Train, 2005, 40(3): 174-180.
3.	Lovering RM, O’Neill A, Muriel JM, et al. Physiology, structure, and susceptibility to injury of skeletal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments. Am J Physiol Cell Physiol, 2011, 300(4): C803-813.
4.	Martinez-Amat A, Marchal-Corrales JA, Rodriguez-Serrano F, et al. Role of alpha-actin in muscle damage of injured athletes in comparison with traditional markers. Br J Sports Med, 2007, 41(7): 442-446.
5.	Capetanaki Y, Milner DJ, Weitzer G. Desmin in muscle formation and maintenance: knockouts and consequences. Cell Struct Funct, 1997, 22(1): 103-116.
6.	Tonino P, Pappas CT, Hudson BD, et al. Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle. J Cell Sci, 2010,123(Pt 3): 384-391.
7.	李世成, 吴维, 杨则宜. 运动后骨骼肌微结构的损伤及修复与蛋白质补充. 中国运动医学杂志, 2004, 23(2): 216-219.
8.	Crameri RM, Aagaard P, Qvortrup K, et al. Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction. J Physiol, 2007, 583(Pt 1): 365-380.
9.	Lieber RL, Friden J. Morphologic and mechanical basis of delayed-onset muscle soreness. J Am Acad Orthop Surg, 2002, 10(1): 67-73.
10.	金其贯, 刘霞, 李淑艳, 等. 反复离心运动致骨骼肌超微结构变化的实验研究. 西安体育学院学报, 2010, 27(4): 453-456.
11.	Martinez-Amat A, Boulaiz H, Prados J, et al. Release of alpha-actin into serum after skeletal muscle damage. Br J Sports Med, 2005, 39(11): 830-834.
12.	张宏, 严隽陶, 徐俊, 等. 骨骼肌纤维类型增龄性变化特征及推拿手法对老龄大鼠骨骼肌纤维的影响. 中国组织工程研究与临床康复, 2007, 11(36): 7149-7152.
13.	王艳国, 严隽陶, 房敏, 等. 推拿对颈椎病患者颈肌疲劳的影响. 江苏中医药, 2008, 40(8): 45-47.
14.	秦渭志, 黄明喜, 陈金昌, 等. 推拿治疗软组织损伤的机制研究. 按摩与导引, 2007, 23(7): 8-9.
15.	田惠林, 赵斌, 刘玉倩, 等. 定量按摩对肌肉损伤修复作用的形态学和生物力学研究. 河北师范大学学报：自然科学版, 2005, 29(2): 213-216.
16.	李军, 魏东明, 文新, 等. 推拿对家兔佐剂性关节炎微循环和血液流变学的影响. 中医药学报, 2010, 38(1): 37-39.
17.	侯懿烜, 柳满然, 张萍, 等. 骨骼肌损伤修复的生理生化机制研究. 激光生物学报, 2011, 20(5): 662-667.
18.	Jimena I, Tasset I, López-Martos R, et al. Effects of magnetic stimulation on oxidative stress and skeletal muscle regeneration induced by mepivacaine in rat. Med Chem, 2009, 5(1): 44-49.
19.	Bär H, Schopferer M, Sharma S, et al. Mutations in desmin’s carboxy-terminal “tail” domain severely modify filament and network mechanics. J Mol Biol, 2010, 397(5): 1188-1198.
20.	Haubold KW, Allen DL, Capetanaki Y, et al. Loss of desmin leads to impaired voluntary wheel running and treadmill exercise performance. J Appl Physiol, 2003, 95(4): 1617-1622.
21.	王瑞元, 苑玉和, 田文秀, 等. 一次力竭性离心运动后大鼠骨骼肌α-actin代谢变化及针刺对其影响. 体育科学, 2002, 22(4): 95-99.
22.	Mahoney DJ, Safdar A, Parise G, et al. Gene expression profiling in human skeletal muscle during recovery from eccentric exercise. Am J Physiol Regul Integr Comp Physiol, 2008, 294(6): R1901-1910.
23.	Negishi S, Li Y, Usas A, et al. The effect of relaxin treatment on skeletal muscle injuries. Am J Sports Med, 2005, 33(12): 1816-1824.

1. 11 Muroya S, Ertbjerg P, Pomponio L, et al. Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle. Meat Sci, 2010, 86(3): 764-769.
2. Zainuddin Z, Newton M, Sacco P, et al. Effects of massage on delayed-onset muscle soreness, swelling, and recovery of muscle function. J Athl Train, 2005, 40(3): 174-180.
3. Lovering RM, O’Neill A, Muriel JM, et al. Physiology, structure, and susceptibility to injury of skeletal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments. Am J Physiol Cell Physiol, 2011, 300(4): C803-813.
4. Martinez-Amat A, Marchal-Corrales JA, Rodriguez-Serrano F, et al. Role of alpha-actin in muscle damage of injured athletes in comparison with traditional markers. Br J Sports Med, 2007, 41(7): 442-446.
5. Capetanaki Y, Milner DJ, Weitzer G. Desmin in muscle formation and maintenance: knockouts and consequences. Cell Struct Funct, 1997, 22(1): 103-116.
6. Tonino P, Pappas CT, Hudson BD, et al. Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle. J Cell Sci, 2010,123(Pt 3): 384-391.
7. 李世成, 吴维, 杨则宜. 运动后骨骼肌微结构的损伤及修复与蛋白质补充. 中国运动医学杂志, 2004, 23(2): 216-219.
8. Crameri RM, Aagaard P, Qvortrup K, et al. Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction. J Physiol, 2007, 583(Pt 1): 365-380.
9. Lieber RL, Friden J. Morphologic and mechanical basis of delayed-onset muscle soreness. J Am Acad Orthop Surg, 2002, 10(1): 67-73.
10. 金其贯, 刘霞, 李淑艳, 等. 反复离心运动致骨骼肌超微结构变化的实验研究. 西安体育学院学报, 2010, 27(4): 453-456.
11. Martinez-Amat A, Boulaiz H, Prados J, et al. Release of alpha-actin into serum after skeletal muscle damage. Br J Sports Med, 2005, 39(11): 830-834.
12. 张宏, 严隽陶, 徐俊, 等. 骨骼肌纤维类型增龄性变化特征及推拿手法对老龄大鼠骨骼肌纤维的影响. 中国组织工程研究与临床康复, 2007, 11(36): 7149-7152.
13. 王艳国, 严隽陶, 房敏, 等. 推拿对颈椎病患者颈肌疲劳的影响. 江苏中医药, 2008, 40(8): 45-47.
14. 秦渭志, 黄明喜, 陈金昌, 等. 推拿治疗软组织损伤的机制研究. 按摩与导引, 2007, 23(7): 8-9.
15. 田惠林, 赵斌, 刘玉倩, 等. 定量按摩对肌肉损伤修复作用的形态学和生物力学研究. 河北师范大学学报：自然科学版, 2005, 29(2): 213-216.
16. 李军, 魏东明, 文新, 等. 推拿对家兔佐剂性关节炎微循环和血液流变学的影响. 中医药学报, 2010, 38(1): 37-39.
17. 侯懿烜, 柳满然, 张萍, 等. 骨骼肌损伤修复的生理生化机制研究. 激光生物学报, 2011, 20(5): 662-667.
18. Jimena I, Tasset I, López-Martos R, et al. Effects of magnetic stimulation on oxidative stress and skeletal muscle regeneration induced by mepivacaine in rat. Med Chem, 2009, 5(1): 44-49.
19. Bär H, Schopferer M, Sharma S, et al. Mutations in desmin’s carboxy-terminal “tail” domain severely modify filament and network mechanics. J Mol Biol, 2010, 397(5): 1188-1198.
20. Haubold KW, Allen DL, Capetanaki Y, et al. Loss of desmin leads to impaired voluntary wheel running and treadmill exercise performance. J Appl Physiol, 2003, 95(4): 1617-1622.
21. 王瑞元, 苑玉和, 田文秀, 等. 一次力竭性离心运动后大鼠骨骼肌α-actin代谢变化及针刺对其影响. 体育科学, 2002, 22(4): 95-99.
22. Mahoney DJ, Safdar A, Parise G, et al. Gene expression profiling in human skeletal muscle during recovery from eccentric exercise. Am J Physiol Regul Integr Comp Physiol, 2008, 294(6): R1901-1910.
23. Negishi S, Li Y, Usas A, et al. The effect of relaxin treatment on skeletal muscle injuries. Am J Sports Med, 2005, 33(12): 1816-1824.

Chinese Journal of Reparative and Reconstructive Surgery

PROMOTING EFFECT OF MASSAGE ON QUADRICEPS FEMORIS REPAIR OF RABBIT IN VIVO

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