EXPRESSION OF β-CATENIN IN ARTICULAR CARTILAGE OF KNEE PRIMARY OSTEOARTHRITIS_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

FANG Xiaomin ¹ , SHI Xiaoqiang ¹ , GAO Zhihui ¹ , BAO Liang ¹ , Wang Shujing ² , JIN Qunhua. ³

1Medicine School of Ningxia Medical University, Yinchuan Ningxia, 750004, P.R.China;;
2the Medical Research Center of Ningxia Medical University;;
3No.3 Department ofOrthopedics, the General Hospital of Ningxia Medical University.;

Corresponding author：

Keywords：

Knee joint ,Primary osteoarthritis ,β-catenin ,Pathogenesis

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Objective To investigate the role of β-catenin in pathogenesis and progression of knee primary
osteoarthritis (OA) by detecting the expression of β-catenin. Methods Between October 2010 and May 2011, 40 cartilage
specimens were collected from adult knee primary OA patients undergoing total knee arthroplasty and 10 cartilage specimens
from adult patients suffering from amputation and femoral condylar fracture. All cartilage samples were taken out from femoral
condylar. The decalcified paraffin-embedded sections were prepared and stained with fast green-safranin O to observe the
degeneration of cartilage, then the modified Mankin scale was used to classify the degeneration. The expression of β-catenin
was detected by the immunohistochemistry staining and Western blot. Results According to the Mankin scale, 10 cases
had normal cartilage, 12 had mild degenerative cartilage, and 28 had moderate to severe degenerative cartilage. The histological
observation showed the mild degenerative cartilage characterized by fissures in the superficial zone of the articular cartilage,
decreased chondrocytes, arrangement disorder, and duplicated tidemark; and the moderate to severe degenerative cartilage
characterized by fissures in the deep zone of the articular cartilage, obviously decreased chondrocytes and cluster, and even fullthickness
cartilage defect. The β-catenin did not expressed in normal articular cartilage; but it expressed in the degenerative
cartilage, and the expression was significantly higher in the moderate to severe degenerative cartilage than in mild degenerative
cartilage (P lt; 0.05). Conclusion β-catenin plays a significant role in the pathogenesis and progression of knee primary OA,
and the mechanism may be the activation of Wnt/β-catenin signaling pathway, which promotes transcri ption of inflammatory
genes and leads to the destruction of articular cartilage.

Citation： FANG Xiaomin,SHI Xiaoqiang,GAO Zhihui,BAO Liang,Wang Shujing,JIN Qunhua.. EXPRESSION OF β-CATENIN IN ARTICULAR CARTILAGE OF KNEE PRIMARY OSTEOARTHRITIS. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(5): 532-535. doi: Copy

1.	Buekwalter JA, Mankin HJ, Grodzinsky AJ. Articular cartilage and osteoarthritis. Instr Course Lect, 2005, 54: 465-480.
2.	管剑龙, 韩星海. 中国骨关节炎十年. 上海: 第二军医大学出版社, 2006: 24-32.
3.	StruttD. Frizzled signaling and cell polarization in Drosophila and vertebrates. Development, 2003, 130(19): 4501-4513.
4.	Luyten FP, Tylzanowski P, Lories RJ. Wnt signaling and osteoarthritis. Bone, 2009, 44(4): 522-527.
5.	中华医学会骨科学分会. 骨关节炎诊治指南 (2007年版). 中华骨科杂志, 2007, 27(10): 793-796.
6.	Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg (Am), 1971, 53(3): 523-537.
7.	Krasnokutsky S, Samuels J, Abramson SB. Osteoarthritis in 2007. Bull NYU Hosp Jt Dis, 2007, 65(3): 222-228.
8.	宋伟, 杨柳, 王富友. 膝关节原发性骨关节炎软骨和软骨下骨病理改变的定量研究. 中国修复重建外科杂志, 2011, 25(12): 1434-1439.
9.	Kawasaki Y, Kugimiya F, Chikuda H, et al. Phosphorylation of GSK-3beta by cGMP-dependent protein kinase II promotes hypertrophic differentiation of murine chondrocytes. J Clin Invest, 2008, 118(7): 2506-2515.
10.	Chen T, Li M, Ding Y, et al. Identification of zinc-finger BED domain-containing 3 (Zbed3) as a novel Axin-interacting protein that activates Wnt/beta-catenin signaling. J Biol Chem, 2009, 284(11): 6683-6689.
11.	Klein TJ, Jenny A, Djiane A, et al. CKIepsilon/discs overgrown promotes both Wnt-Fz/beta-catenin and Fz/PCP signaling in Drosophila. Curr Biol, 2006, 16(13): 1337-1343.
12.	Takahashi M, Tsunoda T, Seiki M, et al. Identification of membrane-type matrix metalloproteinase-1 as a target of the β-catenin/Tcf complex in human colorectal cancers. Oncogene, 2002, 21(38): 5861-5867.
13.	Cong F, Schweizer L, Varmus H. Casein kinase Iepsilon modulates the signaling specificities of dishevelled. Mol Cell Biol, 2004, 24(5): 2000-2011.
14.	Johnson ML, Kamel MA. The Wnt signaling pathway and bone metabolism. Curr Opin Rheumatol, 2007, 19(4): 376-382.
15.	Hwang SG, Ryu JH, Kim IC, et al. Wnt-7a causes loss of differentiated phenotype and inhibits apoptosis of articular chondrocytes vis different mechanisms. J Biol Chem, 2004, 279(25): 26597-26604.
16.	王哲彦, 王文雅, 张柳, 等. 阿仑膦酸钠对IL-1β体外诱导培养的大鼠膝关节软骨细胞影响的实验研究. 中国修复重建外科杂志, 2011, 25(1): 50-55.
17.	Zhu M, Tang DZ, Wu QQ, et al. Activation of β-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult β-catenin conditional activation mice. J Bone Miner Res, 2009, 24(1): 12-21.

1. Buekwalter JA, Mankin HJ, Grodzinsky AJ. Articular cartilage and osteoarthritis. Instr Course Lect, 2005, 54: 465-480.
2. 管剑龙, 韩星海. 中国骨关节炎十年. 上海: 第二军医大学出版社, 2006: 24-32.
3. StruttD. Frizzled signaling and cell polarization in Drosophila and vertebrates. Development, 2003, 130(19): 4501-4513.
4. Luyten FP, Tylzanowski P, Lories RJ. Wnt signaling and osteoarthritis. Bone, 2009, 44(4): 522-527.
5. 中华医学会骨科学分会. 骨关节炎诊治指南 (2007年版). 中华骨科杂志, 2007, 27(10): 793-796.
6. Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data. J Bone Joint Surg (Am), 1971, 53(3): 523-537.
7. Krasnokutsky S, Samuels J, Abramson SB. Osteoarthritis in 2007. Bull NYU Hosp Jt Dis, 2007, 65(3): 222-228.
8. 宋伟, 杨柳, 王富友. 膝关节原发性骨关节炎软骨和软骨下骨病理改变的定量研究. 中国修复重建外科杂志, 2011, 25(12): 1434-1439.
9. Kawasaki Y, Kugimiya F, Chikuda H, et al. Phosphorylation of GSK-3beta by cGMP-dependent protein kinase II promotes hypertrophic differentiation of murine chondrocytes. J Clin Invest, 2008, 118(7): 2506-2515.
10. Chen T, Li M, Ding Y, et al. Identification of zinc-finger BED domain-containing 3 (Zbed3) as a novel Axin-interacting protein that activates Wnt/beta-catenin signaling. J Biol Chem, 2009, 284(11): 6683-6689.
11. Klein TJ, Jenny A, Djiane A, et al. CKIepsilon/discs overgrown promotes both Wnt-Fz/beta-catenin and Fz/PCP signaling in Drosophila. Curr Biol, 2006, 16(13): 1337-1343.
12. Takahashi M, Tsunoda T, Seiki M, et al. Identification of membrane-type matrix metalloproteinase-1 as a target of the β-catenin/Tcf complex in human colorectal cancers. Oncogene, 2002, 21(38): 5861-5867.
13. Cong F, Schweizer L, Varmus H. Casein kinase Iepsilon modulates the signaling specificities of dishevelled. Mol Cell Biol, 2004, 24(5): 2000-2011.
14. Johnson ML, Kamel MA. The Wnt signaling pathway and bone metabolism. Curr Opin Rheumatol, 2007, 19(4): 376-382.
15. Hwang SG, Ryu JH, Kim IC, et al. Wnt-7a causes loss of differentiated phenotype and inhibits apoptosis of articular chondrocytes vis different mechanisms. J Biol Chem, 2004, 279(25): 26597-26604.
16. 王哲彦, 王文雅, 张柳, 等. 阿仑膦酸钠对IL-1β体外诱导培养的大鼠膝关节软骨细胞影响的实验研究. 中国修复重建外科杂志, 2011, 25(1): 50-55.
17. Zhu M, Tang DZ, Wu QQ, et al. Activation of β-catenin signaling in articular chondrocytes leads to osteoarthritis-like phenotype in adult β-catenin conditional activation mice. J Bone Miner Res, 2009, 24(1): 12-21.

Chinese Journal of Reparative and Reconstructive Surgery

EXPRESSION OF β-CATENIN IN ARTICULAR CARTILAGE OF KNEE PRIMARY OSTEOARTHRITIS

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