EFFECT OF HUMAN SALIVA ON WOUND HEALING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

JIA Jinjing ¹ , SUN Ying ² , YANG Huan ¹ , WANG Xinghui ³ , LIU Lei ¹ , ZONG Liang ¹ , HU Hao. ⁴

1Grade 2008 Zonglian, 2Department of Pathology, 3Experiment Center of Function Teaching, 4Departmentof Pharmacology, College of Medicine, Xi’an Jiaotong University, Xi’an Shaanxi, 710061, P.R.China.;

Corresponding author：

Keywords：

Human sal iva, Wound healing ,Rabbit

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To observe the effects of sal iva on impaired raw surface so as to elucidate the possible mechanism in wound heal ing by comparing with Yunnan baiyao. Methods Six wounds (2.5 cm × 2.5 cm in size) were establ ished at both sides on the back of 6 3-month-old adult Japanese rabbits (weighing 2.0-2.5 kg). According to treatment, 36 wounds were randomly divided into 3 groups: wounds were treated with 0.4 mL normal sal ine (blank control group, n=12), 0.5 g Yunnan baiyao powder (Yunnan baiyao group, n=12), and 0.4 mL sal iva of health adult (sal iva group, n=12) for 15 days, respectively. And the general observation of raw surface, the scar formation time, wound healing rate, and histopathology were used to evaluate the effectiveness of sal iva on wound heal ing. Results The wound healing speeds of sal iva group and Yunnan baiyao group were faster than that of blank control group. The wound healing rates of sal iva group were significantly higher than those of blank control group and Yunnan baiyao group at 5, 8, and 11 days after injury (P lt; 0.05). No obvious hemorrhage or necrosis of raw surfaces was observed in sal iva group, and the raw surfaces generally were covered with epidermis at 15 days after injury. The inflammatory cells and microvessel density in sal iva group were significantly less than those of Yunnan baiyao group and control group (P lt; 0.05). Conclusion Sal iva could obviously improve wound heal ing, which is related to its effects on reducing inflammatory cell infiltration, preventing wound infection, accelerating collagen fibers prol iferation, and promoting vessel reconstruction in the process of wound heal ing.

Citation： JIA Jinjing,SUN Ying,YANG Huan,WANG Xinghui,LIU Lei,ZONG Liang,HU Hao.. EFFECT OF HUMAN SALIVA ON WOUND HEALING. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(5): 563-566. doi: Copy

1.	张晓丽, 王济国, 曹美群, 等. 消化系疾病不同舌苔唾液蛋白质组学的初步研究. 中国中医药科技, 2010, 17(4): 336-338.
2.	Carvalho WA, Maruyama SR, Franzin AM, et al. Rhipicephalus (Boophilus) microplus: clotting time in tick-infested skin varies according to local inflammation and gene expression patterns in tick salivary glands. Exp Parasitol, 2010, 124(4): 428-435.
3.	Mamelak AJ, Jackson A, Nizamani R, et al. Leech therapy in cutaneous surgery and disease. J Drugs Dermatol, 2010, 9(3): 252-257.
4.	唐甜, 杨静. 大黄素对家兔实验性皮肤创伤的促愈合作用及其机制. 中国药理学与毒理学杂志, 2006, 20(2): 112-119.
5.	杨超, 邢新, 徐达圆, 等. 脂肪干细胞-透明质酸复合物促进放射性复合损伤创面愈合的初步研究. 中国修复重建外科杂志, 2011, 25(12): 1499-1503.
6.	李钰珑, 吴旭, 张军花, 等. 蜕皮甾酮软膏对家兔创面愈合的影响. 新乡医学院学报, 2008, 25(2): 116-118.
7.	黄康, 陈玉林. 创面愈合评价指标进展. 中国修复重建外科杂志, 2001, 15(2): 126-128.
8.	Min SK, Lee SC, Hong SD, et al. The effect of a laminin-5-derived peptide coated onto chitin microfibers on re-epithelialization in early-stage wound healing. Biomaterials, 2010, 31(17): 4725-4730.
9.	赵飞, 屈景辉, 郑瑞国, 等. 局部氧疗结合近红外线LED照射促进家兔伤口愈合. 第四军医大学学报, 2007, 28(21): 1976-1978.
10.	范伟杰, 杨志明, 罗静聪, 等. 脱细胞羊膜与脱细胞小肠黏膜下层修复猪创伤性皮肤缺损的比较研究. 中国修复重建外科杂志, 2006, 20(2): 155-160.
11.	吴虹桥, 童梅玲, 张敏, 等. 婴儿唾液成分与饮食行为的相关性研究. 江苏大学学报: 医学版, 2009, 19(6): 505-508.
12.	Jensdottir T, Nauntofte B, Buchwald C, et al. Effects of sucking acidic candy on whole-mouth saliva composition. Caries Res, 2005, 39(6): 468-474.
13.	Piangprach T, Hengtrakool C, Kukiattrakoon B, et al. The effect of salivary factors on dental erosion in various age groups and tooth surfaces. J Am Dent Assoc, 2009, 140(9): 1137-1143.
14.	Crooks CV, Wall CR, Cross ML, et al. The effect of bovine colostrum supplementation on salivary IgA in distance runners. Int J Sport Nutr Exerc Metab, 2006, 16(1): 47-64.
15.	Oudhoff MJ, Kroeze KL, Nazmi K, et al. Structure-activity analysis of histatin, a potent wound healing peptide from human saliva: cyclization of histatin potentiates molar activity 1, 000-fold. FASEB J, 2009, 23(11): 3928-3935.
16.	Cohen S. Origins of growth factors: NGF and EGF. J Biol Chem, 2008, 283(49): 33793-33797.
17.	Oudhoff MJ, Bolscher JG, Nazmi K, et al. Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. FASEB J, 2008, 22(11): 3805-3812.
18.	Lee MJ, Kim J, Lee KI, et al. Enhancement of wound healing by secretory factors of endothelial precursor cells derived from human embryonic stem cells. Cytotherapy, 2011, 13(2): 165-178.
19.	Goren I, Allmann N, Yogev N, et al. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol, 2009, 175(1): 132-147.
20.	Hardwicke J, Schmaljohann D, Boyce D, et al. Epidermal growth factor therapy and wound healing—past, present and future perspectives. Surgeon, 2008, 6(3): 172-177.

1. 张晓丽, 王济国, 曹美群, 等. 消化系疾病不同舌苔唾液蛋白质组学的初步研究. 中国中医药科技, 2010, 17(4): 336-338.
2. Carvalho WA, Maruyama SR, Franzin AM, et al. Rhipicephalus (Boophilus) microplus: clotting time in tick-infested skin varies according to local inflammation and gene expression patterns in tick salivary glands. Exp Parasitol, 2010, 124(4): 428-435.
3. Mamelak AJ, Jackson A, Nizamani R, et al. Leech therapy in cutaneous surgery and disease. J Drugs Dermatol, 2010, 9(3): 252-257.
4. 唐甜, 杨静. 大黄素对家兔实验性皮肤创伤的促愈合作用及其机制. 中国药理学与毒理学杂志, 2006, 20(2): 112-119.
5. 杨超, 邢新, 徐达圆, 等. 脂肪干细胞-透明质酸复合物促进放射性复合损伤创面愈合的初步研究. 中国修复重建外科杂志, 2011, 25(12): 1499-1503.
6. 李钰珑, 吴旭, 张军花, 等. 蜕皮甾酮软膏对家兔创面愈合的影响. 新乡医学院学报, 2008, 25(2): 116-118.
7. 黄康, 陈玉林. 创面愈合评价指标进展. 中国修复重建外科杂志, 2001, 15(2): 126-128.
8. Min SK, Lee SC, Hong SD, et al. The effect of a laminin-5-derived peptide coated onto chitin microfibers on re-epithelialization in early-stage wound healing. Biomaterials, 2010, 31(17): 4725-4730.
9. 赵飞, 屈景辉, 郑瑞国, 等. 局部氧疗结合近红外线LED照射促进家兔伤口愈合. 第四军医大学学报, 2007, 28(21): 1976-1978.
10. 范伟杰, 杨志明, 罗静聪, 等. 脱细胞羊膜与脱细胞小肠黏膜下层修复猪创伤性皮肤缺损的比较研究. 中国修复重建外科杂志, 2006, 20(2): 155-160.
11. 吴虹桥, 童梅玲, 张敏, 等. 婴儿唾液成分与饮食行为的相关性研究. 江苏大学学报: 医学版, 2009, 19(6): 505-508.
12. Jensdottir T, Nauntofte B, Buchwald C, et al. Effects of sucking acidic candy on whole-mouth saliva composition. Caries Res, 2005, 39(6): 468-474.
13. Piangprach T, Hengtrakool C, Kukiattrakoon B, et al. The effect of salivary factors on dental erosion in various age groups and tooth surfaces. J Am Dent Assoc, 2009, 140(9): 1137-1143.
14. Crooks CV, Wall CR, Cross ML, et al. The effect of bovine colostrum supplementation on salivary IgA in distance runners. Int J Sport Nutr Exerc Metab, 2006, 16(1): 47-64.
15. Oudhoff MJ, Kroeze KL, Nazmi K, et al. Structure-activity analysis of histatin, a potent wound healing peptide from human saliva: cyclization of histatin potentiates molar activity 1, 000-fold. FASEB J, 2009, 23(11): 3928-3935.
16. Cohen S. Origins of growth factors: NGF and EGF. J Biol Chem, 2008, 283(49): 33793-33797.
17. Oudhoff MJ, Bolscher JG, Nazmi K, et al. Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. FASEB J, 2008, 22(11): 3805-3812.
18. Lee MJ, Kim J, Lee KI, et al. Enhancement of wound healing by secretory factors of endothelial precursor cells derived from human embryonic stem cells. Cytotherapy, 2011, 13(2): 165-178.
19. Goren I, Allmann N, Yogev N, et al. A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. Am J Pathol, 2009, 175(1): 132-147.
20. Hardwicke J, Schmaljohann D, Boyce D, et al. Epidermal growth factor therapy and wound healing—past, present and future perspectives. Surgeon, 2008, 6(3): 172-177.

Chinese Journal of Reparative and Reconstructive Surgery

EFFECT OF HUMAN SALIVA ON WOUND HEALING

Abstract Full text Figures/Tables Video References Cited by

Format

Content