CELL SHEET TECHNOLOGY AND ITS APPLICATION IN BONE TISSUE ENGINEERING_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

CHEN Yali , ZHOU Nuo , HUANG Xuanping.

Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning Guangxi, 530021, P.R.China. Corresponding author: ZHOU Nuo, E-mail: nuozhou@hotmail.com;

Corresponding author：

Keywords：

Bone tissue engineering; Cell sheet technology; Temperature-responsive culture dish; Extracellular matrix

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ObjectiveTo review the progress of cell sheet technology (CST) and its application in bone tissue engineering. MethodsThe literature concerning CST and its application was extensively reviewed and analyzed. ResultsCST using temperature-responsive culture dishes is applied to avoid the shortcomings of traditional tissue engineering. All cultured cells are harvested as intact sheets along with their deposited extracellular matrix. Avoiding the use of proteolytic enzymes, cell sheet composed of the cells and extracellular matrix derived from the cells, and remained the relative protein and biological activity factors. Consequently, cell sheet can provide a suitable microenvironment for the bone regeneration in vivo. With CST, cell sheet engineering is allowed for tissue regeneration by the creation of three-dimensional structures via the layering of individual cell sheets, be created by wrapping scaffold with cell sheets, or be created by folding the cell sheets, showing great potential in tissue engineered bone. ConclusionConstructing tissue engineered bone using CST and traditional method of bone tissue engineering will promote the development of the bone tissue engineering.

Citation： CHEN Yali,ZHOU Nuo,HUANG Xuanping.. CELL SHEET TECHNOLOGY AND ITS APPLICATION IN BONE TISSUE ENGINEERING. Chinese Journal of Reparative and Reconstructive Surgery, 2012, 26(9): 1122-1125. doi: Copy

1.	Yang J, Yamato M, Shimizu T, et al. Reconstruction of functional tissues with cell sheet engineering. Biomaterials, 2007, 28(34): 5033-5043.
2.	Zhou Y, Chen F, Ho ST, et al. Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts. Biomaterials, 2007, 28(5): 814-824.
3.	Okano T, Yamada N, Sakai H, et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly (N-isopropylacrylamide). J Biomed Mater Res, 1993, 27(10): 1243-1251.
4.	Pouliot R, Larouche D, Auger FA, et al. Reconstructed human skin produced in vitro and grafted on athymic mice. Transplantation, 2002, 73(11): 1751-1757.
5.	L’Heureux N, Pâquet S, Labbé R, et al. A completely biological tissue engineered human blood vessel. FASEB J, 1998, 12(1): 47-56.
6.	Nishida K, Yamato M, Hayashida Y, et al. Corneal reconstruction with tissue engineered cell sheets composed of auto ogous oralmuco salepithelium. N Engl J Med, 2004, 351(12): 1187-1196.
7.	Akizuki T, Oda S, Komaki M, et al. Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs. J Periodont Res, 2005, 40(3): 245-251.
8.	Iwata T, Yamato M, Tsuchioka H, et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials, 2009, 30(14): 2716-2723.
9.	Flores MG, Hasegawa M, Yamato M, et al. Cementum-periodontal ligament complex regeneration using the cell sheet technique. J Periodontal Res, 2008, 43(3): 364-371．.
10.	Ohashi K, Yokoyama T, Yamato M, et al. Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets. Nat Med, 2007, 13(7): 880-885.
11.	Shimizu T, Sekine H, Yang J, et al. Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues. FASEB J, 2006, 20(6): 708-710.
12.	Gauvin R, Ahsan T, Larouche D, et al. A novel single-step self-assembly approach for the fabrication of tissue-engineered vascular constructs. Tissue Eng Part A, 2010, 16(5): 1737-1747.
13.	Stock P, Brückner S, Ebensing S, et al. The generation of hepatocytes from mesenchymal stem cells and engraftment into murine liver. Nat Protoc, 2010, 5(4): 617-627.
14.	Yang Z, Jin F, Zhang X, et al. Tissue engineering of cementum/periodontal-ligament complex using a novel three-dimensional pellet cultivation system for human periodontal ligament stem cells. Tissue Eng Part C Methods, 2009, 15(4): 571-581.
15.	Chen CH, Chang Y, Wang CC, et al. Construction and characterization of fragmented mesenchymal-stem-cell sheets for intra muscular injection. Biomaterials, 2007, 28(31): 4643-4651.
16.	Ma D, Zhong C, Yao H, et al. Engineering injectable bone using bone marrow stromal cell aggregates. Stem Cells Dev, 2011, 20(6): 989-999.
17.	Hasegawa M, Yamato M, Kikuchi A, et al. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. Tissue Eng, 2005, 11(3-4): 469-478.
18.	Tsumanuma Y, Iwata T, Washio K, et al. Comparis on of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model. Biomaterials, 2011, 32(25): 5819-5825.
19.	王方, 谢诚, 吴国锋, 等. 组织工程牙周膜细胞片的体内实验研究. 实用口腔医学杂志, 2011, 27( 2): 177-180.
20.	Yang B, Chen G, Li J, et al. Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold. Biomaterials, 2012, 33(8): 2449-2461.
21.	Chen F, Zhou Y, Barnabas ST, et al. Engineering tubular bone constructs. J Biomech, 2007, 40 Suppl 1: S73-79.
22.	Gao Z, Chen FJ, Zhang J, et al. Vitalisation of tubular coral scaffolds withcell sheets for regeneration of long bones: a preliminary study in nude mice. Br J Oral Maxillofac Surg, 2009, 47(2): 116-122.
23.	Nakamura A, Akahane M, Shigematsu H, et al. Cell sheet transplantation of cultured mesenchymal stem cells enhances bone formation in a rat nonunion model. Bone, 2010, 46(2): 418-424.
24.	黄辉, 张森林, 刘向辉, 等. 细胞-膜片复合珊瑚支架修复兔颅骨极限缺损的实验研究. 口腔颌面外科杂志, 2011, 21(4): 256-260.
25.	Tan S, Jing H, Gao H, et al. Complex of dog allogenic decalcified bone matrix and bone marrow stromal cell sheets. 中国组织工程研究与临床康复, 2010, 14(3): 555-558.
26.	陈涛, 王艳辉, 卜令学, 等. 应用细胞片层技术构建功能性组织工程骨的动物实验研究. 华西口腔医学杂志, 2011, 29(4): 442-445.
27.	王玲玲, 李宁毅, 樊功为, 等. 犬骨髓基质细胞片层在构建组织工程骨中的作用. 中国口腔颌面外科杂志, 2011, 9(2): 96-100.
28.	Ma D, Ren L, Liu Y, et al. Engineering scaffold-free bone tissue using bone marrow stromal cell sheets. Orthop Res, 2010, 28(5): 697-702.
29.	Zhou W, Han C, Song Y, et al. The performance of bone marrow mesenchymal stem cell-implant complexes prepared by cell sheet engineering techniques. Biomaterials, 2010, 31(12): 3212-3221.
30.	于淼. BMCs 膜片与种植体复合共培养促进2型糖尿病大鼠骨结合. 西安: 第四军医大学, 2011.
31.	马东洋. 基于骨髓基质干细胞构建无外支架组织工程骨的实验研究. 西安: 第四军医大学, 2010.

1. Yang J, Yamato M, Shimizu T, et al. Reconstruction of functional tissues with cell sheet engineering. Biomaterials, 2007, 28(34): 5033-5043.
2. Zhou Y, Chen F, Ho ST, et al. Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts. Biomaterials, 2007, 28(5): 814-824.
3. Okano T, Yamada N, Sakai H, et al. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly (N-isopropylacrylamide). J Biomed Mater Res, 1993, 27(10): 1243-1251.
4. Pouliot R, Larouche D, Auger FA, et al. Reconstructed human skin produced in vitro and grafted on athymic mice. Transplantation, 2002, 73(11): 1751-1757.
5. L’Heureux N, Pâquet S, Labbé R, et al. A completely biological tissue engineered human blood vessel. FASEB J, 1998, 12(1): 47-56.
6. Nishida K, Yamato M, Hayashida Y, et al. Corneal reconstruction with tissue engineered cell sheets composed of auto ogous oralmuco salepithelium. N Engl J Med, 2004, 351(12): 1187-1196.
7. Akizuki T, Oda S, Komaki M, et al. Application of periodontal ligament cell sheet for periodontal regeneration: a pilot study in beagle dogs. J Periodont Res, 2005, 40(3): 245-251.
8. Iwata T, Yamato M, Tsuchioka H, et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials, 2009, 30(14): 2716-2723.
9. Flores MG, Hasegawa M, Yamato M, et al. Cementum-periodontal ligament complex regeneration using the cell sheet technique. J Periodontal Res, 2008, 43(3): 364-371．.
10. Ohashi K, Yokoyama T, Yamato M, et al. Engineering functional two- and three-dimensional liver systems in vivo using hepatic tissue sheets. Nat Med, 2007, 13(7): 880-885.
11. Shimizu T, Sekine H, Yang J, et al. Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues. FASEB J, 2006, 20(6): 708-710.
12. Gauvin R, Ahsan T, Larouche D, et al. A novel single-step self-assembly approach for the fabrication of tissue-engineered vascular constructs. Tissue Eng Part A, 2010, 16(5): 1737-1747.
13. Stock P, Brückner S, Ebensing S, et al. The generation of hepatocytes from mesenchymal stem cells and engraftment into murine liver. Nat Protoc, 2010, 5(4): 617-627.
14. Yang Z, Jin F, Zhang X, et al. Tissue engineering of cementum/periodontal-ligament complex using a novel three-dimensional pellet cultivation system for human periodontal ligament stem cells. Tissue Eng Part C Methods, 2009, 15(4): 571-581.
15. Chen CH, Chang Y, Wang CC, et al. Construction and characterization of fragmented mesenchymal-stem-cell sheets for intra muscular injection. Biomaterials, 2007, 28(31): 4643-4651.
16. Ma D, Zhong C, Yao H, et al. Engineering injectable bone using bone marrow stromal cell aggregates. Stem Cells Dev, 2011, 20(6): 989-999.
17. Hasegawa M, Yamato M, Kikuchi A, et al. Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model. Tissue Eng, 2005, 11(3-4): 469-478.
18. Tsumanuma Y, Iwata T, Washio K, et al. Comparis on of different tissue-derived stem cell sheets for periodontal regeneration in a canine 1-wall defect model. Biomaterials, 2011, 32(25): 5819-5825.
19. 王方, 谢诚, 吴国锋, 等. 组织工程牙周膜细胞片的体内实验研究. 实用口腔医学杂志, 2011, 27( 2): 177-180.
20. Yang B, Chen G, Li J, et al. Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold. Biomaterials, 2012, 33(8): 2449-2461.
21. Chen F, Zhou Y, Barnabas ST, et al. Engineering tubular bone constructs. J Biomech, 2007, 40 Suppl 1: S73-79.
22. Gao Z, Chen FJ, Zhang J, et al. Vitalisation of tubular coral scaffolds withcell sheets for regeneration of long bones: a preliminary study in nude mice. Br J Oral Maxillofac Surg, 2009, 47(2): 116-122.
23. Nakamura A, Akahane M, Shigematsu H, et al. Cell sheet transplantation of cultured mesenchymal stem cells enhances bone formation in a rat nonunion model. Bone, 2010, 46(2): 418-424.
24. 黄辉, 张森林, 刘向辉, 等. 细胞-膜片复合珊瑚支架修复兔颅骨极限缺损的实验研究. 口腔颌面外科杂志, 2011, 21(4): 256-260.
25. Tan S, Jing H, Gao H, et al. Complex of dog allogenic decalcified bone matrix and bone marrow stromal cell sheets. 中国组织工程研究与临床康复, 2010, 14(3): 555-558.
26. 陈涛, 王艳辉, 卜令学, 等. 应用细胞片层技术构建功能性组织工程骨的动物实验研究. 华西口腔医学杂志, 2011, 29(4): 442-445.
27. 王玲玲, 李宁毅, 樊功为, 等. 犬骨髓基质细胞片层在构建组织工程骨中的作用. 中国口腔颌面外科杂志, 2011, 9(2): 96-100.
28. Ma D, Ren L, Liu Y, et al. Engineering scaffold-free bone tissue using bone marrow stromal cell sheets. Orthop Res, 2010, 28(5): 697-702.
29. Zhou W, Han C, Song Y, et al. The performance of bone marrow mesenchymal stem cell-implant complexes prepared by cell sheet engineering techniques. Biomaterials, 2010, 31(12): 3212-3221.
30. 于淼. BMCs 膜片与种植体复合共培养促进2型糖尿病大鼠骨结合. 西安: 第四军医大学, 2011.
31. 马东洋. 基于骨髓基质干细胞构建无外支架组织工程骨的实验研究. 西安: 第四军医大学, 2010.

Chinese Journal of Reparative and Reconstructive Surgery

CELL SHEET TECHNOLOGY AND ITS APPLICATION IN BONE TISSUE ENGINEERING

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