DUAN Hongyong 1,2 , WU Xin 1 , GU Yongquan 1 , WU Yingfeng 1 , LI Jianxin 1 , CHEN Bing 1 , ZHANG Shuwen 1 , WANG Zhonggao 1 , LIU Zengqing 2
  • 1Department of Vascular Surgery, Xuanwu Hospital &;
  • Institute of Vascular Surgery, Capital Medical University, Beijing,100053, P.R.China;;
  • 2Department of Vascular Surgery, Shanxi Provincial People’s Hospital;
Tissue engineered blood vessels; Porous scaffolds; Decellularization; Extracellular matrix; Porcine
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Objective To investigate the feasibil ity of preparing the porous extracellular matrix (ECM) by use of some chemicals and enzymes to decellularize the porcine carotid artery. Methods The porcine carotid artery was procured, and warm ischemia time was less than 30 minunts. The porcine carotid artery was decellularized with 1% sodium dodecyl sulfate (SDS) for 60 hours to prepare common ECM; then common ECM was treated with 0.25% trypsin (for 6 hours) and 0.3 U/ mL collagenase (for 24 hours) to prepare porous ECM. The common ECM and porous ECM were stained with HE,
Masson’s trichrome, and Orcein to evaluate the histological features. Then the mechanical property, cytotoxicity, and pore size of ECMs were determined. After 4 weeks of subcutaneous implantation in dogs, the histological examination was used for the study. Results Histological observation confirmed that 2 kinds of ECMs were decellularized completely and more porous structure was observed in porous ECM. Scanning electron microscope showed the pores in porous ECM were greater and the length of shorter axis in porous ECM ranged from 5 to 30 μm, the length of longer axis from 40 to 100 μm. The porosity of porous ECM (99.25%) was greater than that of common ECM (91.50%). The burst pressure of porous ECM decreased when compared with common ECM, showing significant difference [(0.154 3 ± 0.012 7) MPa vs [0.305 2 ± 0.015 7) MPa, P  lt; 0.05]. There was no significant difference in suture retention strength between 2 kinds of ECMs (P  gt; 0.05). The cytotoxicity test showed no obvious cytotoxicity in 2 kinds of ECMs. In vivo implantation test showed that the deeper host cells infiltration and more neo-microvessels in porous ECM were observed than in common ECM. Conclusion SDS and some enzymes can be used to prepare porous ECM as the scaffold for tissue engineered blood vessels.

Citation： DUAN Hongyong,WU Xin,GU Yongquan,WU Yingfeng,LI Jianxin,CHEN Bing,ZHANG Shuwen,WANG Zhonggao,LIU Zengqing. PRELIMINARY STUDY ON POROUS SCAFFOLD PREPARED WITH DECELLULARIZED ARTERY. Chinese Journal of Reparative and Reconstructive Surgery, 2010, 24(9): 1052-1057. doi: Copy