To harvest human keratinocyte growth factor (KGF) mimic peptides with Ph.D.-7TM phage display peptide l ibrary. Methods Ph.D.-7TM phage display peptide l ibrary was biopanned for 4 rounds to harvest monoclonal anti-body human KGF and then phage tilter was detected. ELISA detection was performed to detect the binding force of random-selected monoclonal phages, thereafter DNA extracted from phages with better binding activity was sequenced and the Basic BLAST system was appl ied to conduct the sequence similarity and homology analysis. Results After 4 rounds of
biopanning, the titer of phages was increased gradually and the enrichment of specific phage mimic peptides was obtained. The titers of monoclonal phages were up to 2.0 × 1014 pfu/mL according to ELISA detection. According to the absorbance value, the monoclonal phages with better binding activities to certain specific antibodies were sequenced, and 26 base sequences related to the promotion of division and growth were verified, 2 of which were similar to human KGF. Homology sequence analysis revealed that the common sequence of those 26 base sequences was similar to the partial sequences of human KGF. Conclusion The phage mimic peptides resembl ing or related to human KGF DNA can be harvested from Ph.D.-7TM phage display peptide l ibrary, which may be conducive to improve human KGF performance, wound heal ing and the qual ity of tissue engineered skin substitutes.
Citation： ZONG Xianlei,CAI Jinglong,PANG Li,LIU Junli,JIANG Duyin,WANG Wei. SCREENING HUMAN KERATINOCYTE GROWTH FACOTR MIMIC PEPTIDE WITH Ph.D.-7TM PHAGE DISPLAY PEPTIDE LIBRARY. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(2): 183-187. doi: Copy