XIAO Haijun 1 , HOU Chunlin 2 , XUE Feng 1
  • 1Department of Orthopaedics, Fengxian District Center Hospital, Shanghai, 201400, P.R.China;;
  • 2Department of Orthopaedics, Changzheng Hospital, Second Military Medical University.;
Carboxymethylchitosan-carboxymethylcellulose film; Colonic anastomosis; Healing; Adhesion; Rat
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To investigate the effects of carboxymethylchitosan- carboxymethylcellulose (CMCH-CMC) film on the adhesion and heal ing of colonic anastomosis. Methods Sixty-four healthy adult male SD rats was randomly divided into control group and experimental group (n=32). The model of colonic anastomosis was made according to Buckenmaier’ s
method in all rats. The experimental group was treated by wrapping anastomosis with CMCH-CMC film (3 cm × 2 cm) and the control group was not treated. At 7 days and 14 days after operation, the adhesion formation of colonic anastomosis was observed, the tensile strength of the anstomosis was assessed and compared with 6 normal rats, and the hydroxyprol ine (HP) content of the anastomotsis was detected. Results There were 3 deaths in the experimental group and 2 deaths in the control group. The adhesive scores of the experimental group on the 7th and 14th postoperative day [(0.50 ± 0.16) points and (0.45 ± 0.14) points, (P lt; 0.05)] were significantly lower than those of the control group [(1.67 ± 0.15) points and (2.29 ± 0.18) points, (P  lt; 0.05)], (P lt; 0.01). Tensile strength were more marked on the 14th postoperative day than on the 7th postoperative day in the control group (P lt; 0.05), but there was no significant difference between the 7th day and the 14th day in the experimental group. The tensile strength of the
control group and the experimental group on the 14th postoperative day [(178.36 ± 20.10) and (172.74 ± 22.18) mmHg] were respectively higher than those on the 7th postoperative day [(138.67 ± 16.65) and (130.81 ± 18.38) mmHg] (P lt; 0.01). The tensile strength of the control group and the experimental group on the 7th postoperative day were respectively significantly lower than that of the normal rats (P  lt; 0.01). The level of HP in the anastomosis was significantly higher on the 7th postoperative day in the experimental group [(84.47 ± 11.87) μg/mg dried weight] than that of the control group [(55.47 ± 12.89) μg/mg dried weight), (P lt; 0.05)], but there was no significant difference between the experimental group and the control group on the 14th postoperative day [(146.07 ± 14.81) μg/mg dried weight, (137.14 ± 16.81) μg/mg dried weight, (P  gt; 0.05)]. Conclusion The CMCH-CMC film can decrease adhesion the formation of colonic anastomosis, but does not interfere with the heal ing of colonic anastomosis.

Citation： XIAO Haijun,HOU Chunlin,XUE Feng. EFFECT OF CARBOXYMETHYLCHITOSAN-CARBOXYMETHYLCELLULOSE FILM ON COLONIC ANASTOMOSIS HEALING. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(4): 451-455. doi: Copy