YANG Cheng 1 , YAN Rongzeng 2 , GUO Hongyan 1 , GU Xiaoming 1
  • 1Stomatological Center, General Hospital of Chinese People’s Armed Police Forces, Beijing, 100039, P.R.China;;
  • 2Department of Stomatology, Jiangxi General Hospital of Chinese People’s Armed Police Forces.;
Human brain-derived neurotrophic factor; Gene transfection; Fibroblasts; Retrovirus
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Objective To construct human brain-derived neurotrophic factor retroviral vector-pLXSN (hBDNFpLXSN), and to evaluate the bioactivity of hBDNF. Methods The genome mRNA was extracted from embryonic brain tissue of a 5-month-old infant, the hBDNF gene sequence was obtained with RT-PCR technology, and hBDNF-pLXSN constructed in vitro was used to infect the fibroblasts (NIH/3T3). The expression of hBDNF was identfied by the immunohistochemistry method, and the NIH/3T3 and BDNF biological activities were determined by culture of the PC12 cells and dorsal root gangl ia. Results The hBDNF-pLXSN was constructed successfully by sequencing analyses. The infected NIH/3T3 showed positive expression of hBDNF. The infected NIH/3T3 could product hBDNF. Bioactivity of the products could support the PC12
cell survival and neurite growth in the primary cultures of dorsal root gangl ia neurons of mice. Conclusion hBDNF-pLXSN
virus has the abil ity to infect NIH/3T3 and make it expressed and secreted hBDNF with the biological activity. It can be used to treat facial paralysis as a gene therapy.

Citation： YANG Cheng,YAN Rongzeng,GUO Hongyan,GU Xiaoming. CONSTRUCTION OF RETROVIRAL VECTOR WITH HUMAN BRAIN-DERIVED NEUROTROPHIC FACTOR GENE EXPRESSION AND IN THE FIBROBLASTS EXPRESSION. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(5): 588-591. doi: Copy