HOU Nan 1 , CUI Pengcheng 1 , LUO Jiasheng 1 , ZHENG Deyu 2
  • 1Department of Otorlaryngology, Tangdu Hospital, the Fourth Military Medical University, Xi’an Shaanxi, 710038, P.R.China;;
  • 2Department of Otolaryngology, the 3rd Center Hospital of PLA.;
Larynx tissue engineering; Acellular larynx scaffold; Perfusion method; Immersion method; Rabbit
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Objective To compare the difference of preparing the acellular larynx scaffold between perfusion method and immersion method, and find better way to make acellular larynx scaffold for tissue engineering. Methods Twenty 6-month-old male New Zealand rabbits, weighing 2.0-2.5 kg, were divided into perfusion group (n=10) and immersion group (n=10) at random. All the larynxes were excised in a sterile fashion. The acellular larynx scaffold was obtained by perfusion
method and immersion method respectively, and then comparative examinations were performed by the macroscopic
view, histological view, scanning electron microscope (SEM), cartilage vital ity assay and toluidine blue staining. Results
Macroscopic view showed that the larynxes perfused by sodium dodecyl sulphate (SDS) became transparent after 2 hours
of perfusion, but the larynxes immersed by SDS over 16 hours still appeared pink-white. Histology and SEM indicated that
compared with immersion group, perfusion group showed better acellular effect, more ventages and collagen fibers were
retained, no intact cell or nuclei remained in acellular matrix and chondrocytes were still survival. The porosity was 85.39% ± 3.16% in perfusion group and 34.72% ± 4.51% in immersion group, showing significant difference (P  lt; 0.01). The chondrocyte vital ity rate of perfusion group (86.93% ± 1.52%) was higher than that of immersion group (77.73% ± 1.66%), showing significant difference (P  lt; 0.01). Toluidine blue staining showed that the chondrocyte heterochromaty was ber in perfusion group than that in immersion group. Conclusion Compared with immersion method, perfusion method is a better way to construct acellular larynx scaffold because it can achieve better acellular effect and retain chondrocyte vital ity at the greatest extent in the acellular larynx scaffold.

Citation： HOU Nan,CUI Pengcheng,LUO Jiasheng,ZHENG Deyu. A COMPARATIVE STUDY ON PREPARING ACELLULAR LARYNX SCAFFOLD BETWEEN PERFUSING METHOD AND IMMERSING METHOD. Chinese Journal of Reparative and Reconstructive Surgery, 2009, 23(5): 623-626. doi: Copy