JIANG Hua 1 , JIANG Dianming 1 , HUANG Yingru 2 , CHANG Shan 3
  • 1Department of Orthopedics, the First Affiliated Hospital, 2the Traditional Chinese Medical College, Chongqing Medical University, Chongqing, 400016, P.R.China;;
  • 3Department of Orthopedics, the First Affiliated Hospital of Chengdu Medical College.;
Tri pterygium wilfordii; Peri pheral nerve defect; Xenogenous nerve transplantation Immunological rejection; Rat
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Objective To investigate the appropriate concentration of tripterygium wilfordii and immunological rejection of rats’ sciatic nerve allograft with the tripterygium wilfordii’s pretreatment so as to explore tripterygium wilfordii’ s suppression. Methods Sixty SD rats (male, weighing 270-290 g), as sciatic nerve allograft acceptor were randomized into
5 groups (groups A, B, C, D and E, n=12). To repair the sciatic nerve defect of SD rats, the Wistar rats’ sciatic nerve allografts about 15 mm long were used with 24 hours’ soak of different concentrations of tripterygium wilfordii (group A: 200 mg/L, group B: 400 mg/L, group C: 800 mg/L). The control groups (group D: the fresh sciatic nerve allograft from donors; group E: the fresh sciatic nerve allograft from themselves) were establ ished. At different time points after operation, the morphological examinations (the observation of histology, l ight microscope, electron microscope), the detection of myelin basic protein’s (MBP) content and the analyses of CD4+ and CD8+ T cells on the allografts in the acute phase were performed Results There was no significant difference in morphology among groups A, B and C, the adhesions between allografts and connective tissue were milder than that of group D, and the allografts’ morphous and the inflammatory cell infiltration were better than that of group D. The degeneration of myel in sheath was observed at different levels and there was no significant difference between group B and group E (P  gt; 0.05). There was a significant difference in immunological rejection between groups A, B, C and group D (P  lt; 0.05). Conclusion Tripterygium wilfordii can effectively suppress the acute immunological rejection in the early stage after operation, and protect the myel in sheath to a certain extent.

Citation： JIANG Hua,JIANG Dianming,HUANG Yingru,CHANG Shan. EXPERIMENTAL STUDY ON DEMYELINATION AND IMMUNOLOGICAL REJECTION OF RATS’SCIATIC NERVE ALLOGRAFT WITH TRIPTERYGIUM WILFORDII’S PRETREATMENT. Chinese Journal of Reparative and Reconstructive Surgery, 2008, 22(11): 1378-1383. doi: Copy