CHENG Biao 1 , LIU Hongwei 1 , FU Xiaobing 2 , SHENG Zhiyong 2 , SUN Tongzhu 2
  • 1 Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou Guangdong, 510010, P.R. China;;
  • 2 Wound Healing and Cell Biology Laboratory, Institute for Basic Research, Trauma Center of Postgraduate Medical College, General Hospital of PLA, Beijing， 100853, P.R. China;
Diabetic; Platelet-derived growth factor; Wound healing; Extracellular signal-regulated kinase
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Objective To investigate the possible signaling mechanisms by which recombinant human plateletderived growth factor (rhPDGF) accelerated healingof cutaneous wound in diabetic rats. Methods Four full-thickness skin woundswere incised in the back of 26 male Wistar diabetic rats. The wounded rats were divided into 3 groups (7 or 8 rats each group). One group without treatmentwas used as a control, and the other 2 groups were treated with rhPDGF at a dose of 7.0 μg/cm2 wound or vehicle (DMSO/09% NaCl, vol/vol 1∶1) from 1 to14 days. The wound healing was evaluated by the measurements of the wound volume and area. Immunofluorescent and immunohistochemical staining were used to examine the phosphorylation of extracellular signalregulated kinase 1/2 (ERK1/2) andthe expression of proliferative cell nuclear antigen (PCNA), respectively. Results Granulation tissue appeared in the bed of wound after injury. The number of blood capillary buds and fibroblasts was greater in the rhPDGF-treated group than that in the other 2 groups. A lot of inflammatory cells infiltration and collagen deposition were observed in the wound. The wound-volume in the rhPDGF-treated group was smaller than that in control group (P lt;0.05). The reepithelialization rate in rhPDGF-treated group was higher than that inthe other 2 groups at 7 days after injury (P lt;0.05). The expression of PCNA in reparative cells was higher in rhPDGF-treated group than in control group or vehicle-treated group at 3,7 days after injury(P lt;0.05). The phosphorylation of ERK1/2 was ber in rhPDGF-treated group than that in control group or vehicle group at 7 and 14 days after injury(P lt;0.05). Conclusion These results suggest that rhPDGF accelerates wound healing and improves healing quality by increasingthe phosphorylation of ERK1/2.

Citation： CHENG Biao,LIU Hongwei,FU Xiaobing,SHENG Zhiyong,SUN Tongzhu. Recombinant Human Platelet-derived Growth Factor-Enhances Repair of Cutaneous Full-thickness-Excision by Increasing the Phosphorylation-of Extracellular Signal-regulated Kinase-in Diabetic Rat. Chinese Journal of Reparative and Reconstructive Surgery, 2006, 20(11): 1093-1098. doi: Copy