• Department ofOrthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, P. R. China;
Glial cell line-derived neurotrophic factor; Transfection; Retroviral vectors; Identification
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Objective To identify glial cell line-derived neurotrophic factor (GDNF) recombinant retroviral vector and to establish its packaging cell line PA317. Methods PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes. The recombinant retroviral particles were then harvested from culture media of G418 resistant transfected cells and analyzed using RT-PCR. Virus titers in supernatants were investigated. Results Sequencing date indicated that GDNF gene was exactly identical to the sequence in the GeneBank. PA317 cells were transfected with recombinant retroviral vector pLXSN-GDNF using liposomes, and virus titers insupernatants harvested from culture media of G418 resistant transfected cells were 104-105 CFU/ml. Conclusion Packaging cell line PA317/pLXSN-GDNF was established.

Citation： ZHANG Wenjie,LI Bingcang,REN Xianjun,et al.. IDENTIFICATION OF GLIAL CELL LINEDERIVED NEUROTROPHIC FACTOR RECOMBINANT RETROVIRAL VECTOR AND ESTABLISHMENT OF ITS PACKAGING CELL LINE PA317. Chinese Journal of Reparative and Reconstructive Surgery, 2006, 20(11): 1119-1123. doi: Copy