LI Hongwei 1 , ZHANG Donghua 1 , WEN Yuming 2 , et al. 2
  • Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology of Nanjing Medical University Jiangsu, Nanjing,210029,P.R.China;
Tissue engineered oral mucosa; Hetero transplantation; Application
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To investigate the growth of the tissue engineered mucosa after its heterotransplantation. Methods The epithelial cells and fibroblasts were isolated from a postoperative tissue of the 3month patient with labial
cleft. The epithelial cells and fibroblasts were separately seeded on the polylactic／glycolic acid copolymer membrane, and then they were exposed to the air-liquid interface. Seven volunteer patients, whose traumatic beds were repaired with the tissue engineered oral mucosa. The biopsy tissue from one of the seven patients was observed under light icroscope 18 and 30 days after transplantation, respectively. Results The tissue engineered oral mucosa having 5-6 layers anticytoeratin staining positively cells in the epithelial layer and 3-7 layers anti-Cytoeratin staining negatively cells in the subepithelial layer grew well after the he terotransplantation. No differ ence could be found between the transplanting and normal areas. At 18 days, the epithelial layer and lamina propria grew well and the fibroblasts were found; at 30 days, collagen was obviously observed. The structures in both the transplanting and the normal areas were similar. Conclusion The tissue engineered oral mucosa can grow well after the heterotransplantation.

Citation： LI Hongwei,ZHANG Donghua,WEN Yuming,et al.. GROWTH OF COMPOUND LAYER TISSURE ENGINEERED ORAL MUCOSA AND ITS CLINICAL APPLICATION IN HETERO-TRANSPLANTATION. Chinese Journal of Reparative and Reconstructive Surgery, 2006, 20(2): 177-180. doi: Copy