LU Feng , Gao Jianhua.
  • Department of Plastic Surgery, Nanfang Hospital, Southern Medical University(the First Military Medical University),Guangzhou Guangdong,510515， P.R. China.;
Fas gene; Adenovirus; Keloid; Gene therapy; In vitro
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Objective To replace dysfunctional Fas gene and reconstruct the blocked Fas signal by using two kinds of prepared recombinantAdenovirus which have human Fas gene. Methods After the keloids derived from fibroblasts were infected by the Adenovicus, the expressions of Fas protein before the exposure and after the exposure was compared. Then the function of the newly produced Fas protein was detected. Results The highly improve expression of Fas protein in the infected keloid derived fibroblasts was detected. Obvious apoptosis was also detected in the infected keloid derived from fibroblasts under the condition of exposing to FasMcab. Conclusion ①The recombinant Adenovirus with Fas gene can transfect the Fas gene into keloidderived fibroblasts and highly improved the expression of Fas protein. The newly expressed Fas gene can reconstruct the blocked Fas signal. ②Ad-Fas(B) has better therapeutic effect in vitro gene therapy. ③ The correlation between keloid and Fas gene was further proved and it may pave the way for further gene therapy in keloid .

Citation： LU Feng,Gao Jianhua.. EXPERIMENTAL GENE THERAPY OF KELOID IN VITRO USING RECOMBINANT ADENOVIRUS CODING FOR FAS GENE. Chinese Journal of Reparative and Reconstructive Surgery, 2005, 19(1): 35-38. doi: Copy