• Department of Otorhinolaryngology，Tang Du Hospital of the Forth Military Medical University，Xi’an Shaanxi，710038，P. R. China;
Tissue engineering; Marrow mesenchymal stem cells bank; Cryopreservation
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To investigate the possibility of establishing the human bone marrow mesenchymal stem cells (hMSCs) bank as to provide an alternative source for the seed cells of tissue engineering. Methods The cell surface antigensof the purified, expanded hMSCs and the ones following cryopreservation were detected by flow cytometry, cultured in a special medium to induce the ostoegenic and chondrocytic- differentiation. Morphology was studied by light and electronic microscopes. The detection of alkaline phosphatase, collagen type Ⅰ, osteocalcin, and collagen type Ⅱ were also performed by immunochemistry and molecular biology.Results The phenotype and expansion possibility of hMSCs after cryopreservation were remained. It could expand for 10 generations. The doubling time was 40 h.Conclusion The bank of hMSCs is inipiently established and can provide eligible seed cells for tissue engineering.

Citation： PANG Yonggang,CUI Pengcheng,CHEN Wenxian,et al.. INCIPIENT ESTABLISHMENT OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS BANK. Chinese Journal of Reparative and Reconstructive Surgery, 2005, 19(7): 578-582. doi: Copy