• Department of Plastic Surgery, the First Affiliated Hospital of Anhui University of Medical Science. Hefei Anhui, P.R. China 230022;
Tissue engineering scaffold materials; Marrow mesenchymal stem cells; Biocompatibility
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Objective To investigate the biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stell cells (MSCs).
Methods MSCs were inoculated to p(3HB-co-3HH), and then cultured for 2-4 weeks in vitro and embedded for 2 weeks in vivo. The growth, proliferation, morphology and phenotype properties of MSCs were observed by use of phase contrast microscope, electron microscope, HE staining and staining of type Ⅰ collagen. Results p(3HB-co-3HH) hadgood compatibility. The inoculated MSCs could be well-distributed, attached well and obtain the phenotype of MSCs in p(3HB-co-3HH). After osteogenic inducer were added, MSCs differentiated to osteoblasts and secreted matrix. Type Ⅰ collagen was stained positively by immunohistochemical techenique. Conclusion The above results demonstrate that there is satisfactory biocompatibility betweenp(3HB-co-3HH) and MSCs.

Citation： ZHAO Yu,HU Ping,LU Yingling,et al.. BIOCOMPATIBILITY OF p(3HB-co-3HH) AND MARROW MESENCHYMAL STEM CELLS. Chinese Journal of Reparative and Reconstructive Surgery, 2004, 18(2): 104-107. doi: Copy