• Institute of Space Medico Engineering. Beijing, P. R. China 100094.;
Simulated microgravity; Rats; Cardiac myocytes; Three-dimensional culture
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Objective To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity. Methods Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirredin spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed underinverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay. Results Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLAcardiac myocytes was found in some areas. Conclusion The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.

Citation： YANG Fen,LI Yinghui,NIE Jielin,et al.. PILOT STUDY OF NEONATAL RAT CARDIAC MYOCYTES CULTURED FOR THREE-DIMENSIONAL MODELING IN SIMULATED MICROGRAVITY. Chinese Journal of Reparative and Reconstructive Surgery, 2004, 18(2): 119-122. doi: Copy