• Department of Orthopedic Surgery, the First Affiliated Hospital, China Medical University, Shenyang Liaoning, 110001, P. R. China ;
Tissue engineering; Osteoblasts; Vascular endothelial cells; Biological behavior
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Objective To study the biological behavior of osteoblast and vascular endothelial cell culture. Methods The osteoblasts and vascular endothelial cells were obtained from calvarial bone and renal cortox of 2-week rabbits respectively. The experiment were divided into group A (osteoblasts), group B (vascular endothelial cells) and group C(co-cultured osteoblasts and vascular endothelial cells). The cells were identified with cytoimmunochemical staining. The cellular biological behavior and compatibilitywere observed under inverted phase contrast microscope and with histological staining. The cells viability and alkaline phosphatase(ALP) activity were measured. Results The cytoimmunochemical staining showed that the cultured cells were osteoblasts and vascular endothelial cells .The cellular compatibility of osteoblasts and vascular endothelial cells was good. The ALP activity was higher in group C than in group A and group B(P＜0.01), and it was higher in group A than in group B(P＜0.05). In group C, the cellproliferation were increased slowly early, but fast later. Conclusion Thecellular compatibility of osteoblasts and vascular endothelial cells were good. The vascular endothelial cells can significantly increased the osteoblast viability and ALP activity,and the combined cultured cells have greater proliferation ability.

Citation： RUI Gang,WANG Zhesheng,LU gang,et al.. STUDY ON BIOLOGICAL BEHAVIOR OF OSTEOBLAST AND VASCULAR ENDOTHELIAL CELLCULTURE. Chinese Journal of Reparative and Reconstructive Surgery, 2004, 18(4): 327-330. doi: Copy