• Institute of Biomedical Engineering;;
  • Chinese Academy of Medical Sciences &;
  • Peking Union Medical College. Tianjin;;
  • P. R. China 300192;;
Tissue engineering dermis; Collagen; Chondrontin sulfate; Fibroblast
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective　To const ruct art ificial derm is on co llagen2chondront in sulfate (CS) scaffo ld. Methods　
Co llagen w as compounded from CS and 1-ethyl-3-(13-dimethyl am inop ropyl) carbodiim ide (EDC) used as a cro sslinker. Physical and chem ical p ropert ies of the scaffo ld w ere characterized by elect ron spect ro scopy fo r chem ical analysis (ESCA ) , scanning elect ron m icrograph (SEM ) , HE staining, and mechanical p roperty test. Derm is fibroblasts w ere iso lated from human embryo and w ere cultured on the scaffo lds. Th rough h isto logical test ing, immunoh istochem ical test ing and biochem ical p roperty test ing, the p roperty of co llagen-CS art ificial derm is w as compared w ith that of colla gen spongy art ificial derm is. Results　Co llagen-CS had th ree2dimension st ructure w ith po rous. Compared w ith co llagen scaffo ld, themechanical p roperty of co llagen2CS scaffo ld imp roved. There w eremo re po lar group s on the surface of co llagen-CS scaffo ld. The fibroblasts on the co llagen-CS scaffo ld grew w ell, and art ificial derm is w as const ructed. Conclus ion 　Co llagen-CS art ificial derm is has mo re excellent bio logical and mechanical p ropert ies. F ibroblasts at tach and p ro liferate bet ter on co llagen2CS scaffo ld than on co llagen scaffo lds.

Citation： LIU Ling rong,ZHANG Li hai,MA Dong rui,et al.. CONSTRUCTION OF ARTIFICIAL DERMIS ON COLLAGEN-CHONDRONTIN SULFATE SCAFFOLD CROSSLINKED BY 1-ETHYL-3-(13-DIMETHYL AMINOPROPYL) CARBODIIMIDE. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(2): 83-88. doi: Copy