• Burn Center;;
  • Ruijin Hospital;;
  • Shanghai Second Medical University. Shanghai;;
  • P. R. China 200025;;
Tissue engineering skin; Acellular dermal matrix; Attachment; Fibroblast
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Objective To compare the attachment and growth of fibroblasts on the different porcine accellular dermal matrix (ADM) so as to find the suitable scaffold for tissue engineering skin. Methods Fibroblasts (5×10 5) were seeded on 4 kinds of ADMs which were crosslinked with glutaraldehyde, uncrosslinked, crosslinked with glutaraldehyde and removed basement membrane, corsslinked with glutaraldehyde and then meshed. The same density fibroblasts were seeded on petri dish as a control. Cell count was done on the 1st, 3rd, 5th days after seeding. The at tachment of fibroblasts on ADM sw as observed by HE staining. Results　The grow th and at tachment of fibroblasts on cro sslinked and non2meshed ADM increasedmarkedly w hen compared w ith the o thers. There w as no obvious difference betw een the group s of w ith o r w ithout basement membrane. Conclus ion　The above results indicate that non2meshed and co rsslinked w ith glutaraldehyde ADM ismo re suitable fo r the at tachment and grow th of fibroblasts than the o thers
and that the modified ADM can be used fo r the scaffo ld of t issue engineering skin.

Citation： LIU Yan,ZHANG Xiong,YAO Min,et al.. EFFECT OF PORCINE ACELLULAR DERMAL MATRIX ON THE ATTACHMENT AND GROWTH OF HUMAN SKIN FIBROBLAST. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(2): 97-99. doi: Copy