• Department of Burn;;
  • Changhai Hospital;;
  • Second Military Medical University. Shanghai;;
  • P. R. China 200433;;
Tissue engineering skin; Vascularization; Acellular dermal matrix; Fibroblasts
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Objective To investigate the possible mechanism of the fibroblasts inducing the vascularization of dermal substitute. Methods Fibroblasts were seeded on the surface of acellular dermal matrix and cultivated in vitro to construct the living dermal substitute. The release of interleukin 8 (IL 8) and transfonming growth factor β 1(TGF β 1) in culture supernatants were assayed by enzyme linked immunosorbent assay, the mRNA expression of acid fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) were detected by RT-PCR. Then, the living substtute was sutured to fullth ickness excised wound on BALB ouml;C m ice, and the fate of fibroblast w as observed by using in situ hybridizat ion. Results　Fibroblasts cultured on acellular dermalmat rix p ro liferated and reached a single2layer confluence. Fibroblasts could secret IL 28 (192. 3±15. 9) pg ouml;m l and TGF-B1 (1. 105±0. 051) pg ouml;m l. There w as the mRNA exparession of aFGF and bFGF. Fibroblasts still survived and proliferated 3 weeks after graft ing. Conclusion　Pept ides secreted by fibroblasts and its survival after graft ing may be relat ive to the vascularizat ion of the dermal subst itute.

Citation： XIAO Shi chu,XIA Zhao fan,YANG Jun,et al.. MECHANISM OF THE FIBROBLAST INDUCING THE VASCULARIZATION OF DERMAL SUBSTITUTE. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(2): 100-103. doi: Copy