OBJECTIVE: To construct a plasmid which has a reporter gene for exploring the role of human telomerase reverse transcriptase(hTRT) in in-vitro cell cultivation. METHODS: hTRT was cut by restricted enzyme from plasmid pGRN145 and inserted to plasmid pEGFP-C1 (enhanced green fluorescent protein). RESULTS: Restricted enzyme analysis and DNA sequencing showed that the sequence of the pEGFP -hTRT transgenic plasmid was correct. CONCLUSION: The recombinant vector pEGFP-hTRT has been successfully constructed, and it can be used as a transgenic plasmid in generating immortalized cell lines.
Citation： ZHOU Haiwen,ZHOU Zengtong,YANG Wenjun,et al.. CONSTRUCTION OF RECOMBINANT pEGFP/hTRT PLASMID. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(3): 230-232. doi: Copy