• Laboratory of Medical Molecular Biology;;
  • School of Basic and Forensic Medicine;;
  • Sichuan University. Chengdu;;
  • Sichuan;;
  • P. R. China 610041. E mail:quyi712002@yahoo.com.cn;;
Human umbilicus vein endothelial cell; Human telomerase reverse transcriptase; mRNA; Transfection; Proliferation life span
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

OBJECTIVE: To elongate the proliferation life-span of human umbilicus vein endothelial cell (HUVEC). METHODS: We synthesized the human telomerase reverse transcriptase mRNA (hTERT mRNA) by in vitro transcription, then transferred the hTERT mRNA into HUVEC in quicent stage by lipofect introduction. RESULTS: Telomerase expressed transiently in HUVEC, and the cell life-span was elongated for 7 population doublings. CONCLUSION: Telomerase can be reconstructed controllably and transiently in HUVEC by hTERT mRNA introduction, this method has the potential to be used to elongate the lifespan of cells cultured in vitro.

Citation： QU Yi,WANG Zheng rong,Huang Xiang,et al.. EFFECT OF HUMAN TELOMERASE REVERSE TRANSCRIPTASE mRNA INTRODUCTION ON THE GROWTH OF HUMAN UMBILICUS VEIN ENDOTHELIAL CELL. Chinese Journal of Reparative and Reconstructive Surgery, 2003, 17(5): 411-413. doi: Copy