Objective To study the biological characteristic and potential of chondrocytes grafting cultured on fascia in repairing large defect of articular cartilage in rabbits. Methods Chondrocytes of young rabbits were isolated and subcultured on fascia. The large defect of articular cartilage was repaired by grafts of freeze-preserved and fresh chondrocytes cultured on fascia, and free chondrocytes respectively; the biological characteristic and metabolism were evaluated by
macroscopic, histological and immunohistochemical observations, autoradiography method and the measurement of nitric oxide content 6, 12, 24 weeks after grafting. Results The chondrocytes cultured on fascia maintained normal growth feature and metabolism, and there was no damage to chondrocytes after cryopreservation; the repaired cartilage was similar to the normal cartilage in cellular morphology and biological characteristics. Conclusion Chondrocytes could be cultured normally on fascia, which could be used as an ideal carrier of chondrocytes.

Citation： YANG Guiyong,LU Shibi,ZHANG Boxun,et al.. EXPERIMENTAL STUDY ON REPAIR OF ARTICULAR CARTILAGE DEFECT IN LARGE AREA WITH CHONDROCYTES CULTURED ON FASCIA. Chinese Journal of Reparative and Reconstructive Surgery, 2002, 16(4): 223-227. doi: Copy