Objective To investigate the outcome and histological changes of transplantation of acellular xeno-dermis combined with suspended keratinocytes.Methods Forty-two nude mice with full-thickness skin defect on the back were randomly divided into 2 groups, then acellular xeno-dermisand and suspended keratinocytes were adopted to cover the skin defect in the experimental group, pure suspended keratinocytes in the control group. The area of wound healing was calculated2, 3 and 5 weeks after transplantation, and the rates of wound contraction werealso calculated,and biopsy for histological examination was performed 3, 6and 12 weeks after transplantation. Results Compared with the experimental group,the control group showed delayed wound healing (P＜0.05), intensive wound contraction (P＜0.05), poor durability, elasticity, and cosmetic appearances as well asdisordered collagen fibers. In contrast, it was observed that the proliferationof collagen fibers was regularly organized, with no obvious acute immuno-rejection responses in the experimental group. Conclusion The composite transplantation of acellular xenodermis and suspended keratinocytes could promote the woundhealing with a satisfactory outcome.

Citation： LIU Wang,HUAN Jingning,CHEN Yulin,et al.. EXPERIMENTAL STUDY ON TRANSPLANTATION OF ACELLULAR XENO-DERMIS AND SUSPENDED KERATINOCYTES. Chinese Journal of Reparative and Reconstructive Surgery, 2002, 16(4): 248-251. doi: Copy