• Department of Orthopedic Surgery;;
  • Third Hospital of Hebei Medical University. Shijiazhuang Hebei;;
  • P.R.China;;
  • 050051;;
Gene transfer; Gene expression; Exogenous gene; Sciatic nerve
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OBJECTIVE To probe the possibility of direct transfer of exogenous gene into peripheral nerve and its following expression in vivo. METHODS The PCMV beta plasmid containing cytomegalovirus (CMV) promoter and Escherichia Coli (E. Coli), beta-Galactosidease (beta-Gal) structural gene (lacZ gene) was constructed and injected into the rabbit sciatic nerve. The control group was injected PBS solution. The injected nerves were sampled and tested by beta-Gal enzyme activity assay of the 5-bromo-4-chloro-3-indolyl-beta-D-galactoside and beta-Gal histochemical stain. RESULTS In the control group, no beta-Gal enzyme activity was detected in the different stages after operation, and beta-Gal histochemical stains showed positive. In the experimental group, enzyme activity could be detected from 2 days to 30 days after operation, and the histochemical stains showed negative. CONCLUSION The exogenous gene can be transferred into peripheral nerve and expressed with bioactivity, thus the gene therapy to accelerate the recovery of nerve is practical.

Citation： ZHANG Shi qiang,ZHANG Jing qi,XU Xiu lan,et al.. DIRECT GENE TRANSFER INTO RABBIT PERIPHERAL NERVE IN VIVO. Chinese Journal of Reparative and Reconstructive Surgery, 1999, 13(4): 206-208. doi: Copy