• Shanghai Institute of Biochemistry;;
  • the Chinese Academy of Sciences. Shanghai;;
  • P.R. China 200031;;
Gene therapy; Wound healing; Fibroblasts; Epidermal growth factor
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

OBJECTIVE This paper aims to explore the new method of continuous delivery of epidermal growth factor to wounds by transfected fibroblasts to promote wound repair. METHODS It was constructed a novel chimeric expression plasmid in which the biologically active portion of the human epidermal growth factor (EGF) gene was fused in-frame to the human granulocyte colony-stimulating factor signal sequence. RESULTS Clonally selected human fibroblasts transfected with this construct could secrete biologically active EGF. After the transplantation of irradiated gene-transfected fibroblasts suspended in fibrin glue to murine full-thickness wounds, EGF could be demonstrated for at least seven days in the wounds, slowly decreasing from initially 470 ng/L to 140 ng/L in 7 days. No EGF was found in the wound at 14 days. CONCLUSION A single application of irradiated EGF gene transfected fibroblasts to wounds can continuously deliver the transgene in vivo and can be used to administer drugs to the wound bed during the crucial first seven days of wound-healing.

Citation： CAO Lei,Felicia Rosenthal,JIAO Xiang yang,et al.. CONTINUOUS DELIVERY OF EPIDERMAL GROWTH FACTOR TO WOUNDS IN VIVO BY GENETICALLY MODIFIED FIBROBLASTS TRANSFECTED WITH A NOVEL CHIMERIC CONSTRUCT. Chinese Journal of Reparative and Reconstructive Surgery, 1999, 13(5): 263-269. doi: Copy

Copyright © the editorial department of Chinese Journal of Reparative and Reconstructive Surgery of West China Medical Publisher. All rights reserved