In order to study the expression change of insulin-like growth factor-1 (IGF-1) and its receptor genes in different generations of tendon cell in culture, Dig-labeled synthesized oligonucleotide probes were used to detect the mRNA expression in primary, 6th and 13th generation of tendon cell. The results showed that IGF-1 receptor mRNA was expressed in all of the 3 above generation tendon cells. IGF-1 mRNA was expressed only in primary and 6th generation cells. Tendon cell of 13th generation did not express IGF-1 mRNA. It might suggest that the absence of IGF-1 mRNA expression be one of the causes which led to the decrease of reproductive ability of 13th generation tendon cell.
Citation： Xiang Zhou,Yang Zhiming,Xia Qinjie et al.. THE EXPRESSION OF IGF 1 AND ITS RECEPTOR IN CULTURED TENDON CELL. Chinese Journal of Reparative and Reconstructive Surgery, 1997, 11(6): 369-371. doi: Copy