It has been proved that the bovine seminal plasma contains rich source of NGF around 0.1mg of pure NGF can be isolated from 10ml bovine seminal plasma. Modifying Gregory s method, we first successfully obtained the low molecular weight form of bovine NGF in China.We chose the dorsal root sensory ganglia (DRG) of embryonic chicken as a cultured nerve tissue, the NGF purified from seminal plasma is added to cultural plate with 96 holes. The cultural process was without plus serum and showed the high biological activity. It was found that this method has the advantages of simple technique, satisfactory result. It is an ibeal method for assaying the biological effect of NGF.
Citation： Fang Huang,Lou Yongxiang,et al.. THE DETERMINATION OF BIOLOGICAL EFFECT OF NERVE GROWTH FACTOR PURIFIDED FROM BOVINE SEMINAL PLASMA. Chinese Journal of Reparative and Reconstructive Surgery, 1993, 7(1): 32-34. doi: Copy