李文博 1 , 李筱荣 1 , 鲁振宇 2 , 张云山 3 , 东莉洁 3 , 王飞 1 , 董荣 1
李筱荣, Email: xiaorli@hotmail.com
Embryonic stem cells/physiology; Cell differentiation/physiology; MicroRNAs/physiology
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Objective  To observe the expression of miR-204 and 211 human embryonic stem cells (hESCs) differentiated into retinal pigment epithelial (RPE) cells. Methods  RPE cells were derived from hESCs by natural differentiation method, and were identified. miRNA expression profiles and real-time polymerase chain reaction (RT-PCR) of miR-204 and 211 were generated from the following groups: hESCs, hESCs-derived cells containing pigmented foci, hESCs-derived RPE cells and human fetal RPE (hfRPE) cells. Results  miRNA-204 was continuously upregulated throughout the entire differentiation process of hESCs to RPE cells. It increased 5.026 times in hESCs-derived cells containing pigmented foci compared to hfRPE cells; it was increased 3.337 times in hESCs-derived RPE cells compared to hESCs-derived cells containing pigmented foci; it increased 13.574 times in hfRPE cells compared to hESCs-derived RPE cells. miR-211 does not change during differentiation from hESC to RPE, but it increased 44.333 times in hESCderived RPE cells compared to hfRPE cells. miR-211 was the biggest difference in the miRNA expression pattern. In four cell types of hESCs, hESCs-derived cells containing pigmented foci, hESCs-derived RPE cells and hfRPE cells, RT-PCR showed the levels of miR-204 were 91.81 plusmn;4.43, 2263.09 plusmn;206.39, 5996.80 plusmn;235.42, and 171676.45 plusmn;999.82 respectively. miR-204 was significantly increased during the whole course (t=18.22, 20.66, 279.38;P＜0.001). The levels of miR-211 were 2.23 plusmn;0.31, 129.33 plusmn;3.75, 125.7592 plusmn;4.78, and 16682.00 plusmn;352.97 respectively. miR-211 was significantly increased from hESCs to cells containing pigmented foci and from hESCs-derived RPE cells to hfRPE (t=58.58, 81.24; P＜0.001). Conclusion  There is a continuous change of miR-204 and 211 in differentiation of RPE cells from hESCs.

Citation： 李文博,李筱荣,鲁振宇,张云山,东莉洁,王飞,董荣. Expression of miR-204 and 211 in human embryonic stem cells differentiated into retinal pigment epithelial cells. Chinese Journal of Ocular Fundus Diseases, 2013, 29(1): 72-75. doi: Copy