Objective To observe the effect of high glucose on the expression of activating transcription factor 4 (ATF4) in cultured retinal M uuml;ller glia cells. Methods The retinal tissue of Sprague-Dawley (SD) rats was collected, and M uuml;ller cells were isolated and cultured. The glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) of M uuml;ller cells were identified by streptavidin-biotin-peroxidase complex. Cultured rat M uuml;ller cells were divided into control group (5.5 mmol/L glucose), group A (20 mmol/L glucose), group B (30 mmol/L glucose) and group C (40 mmol/L glucose). ATF4 protein expressions in M uuml;ller cells of four groups were measured by Western blot four days after cultured. Results GFAP and GS expressed in more than 95% of M uuml;ller cells. Over 95% of M uuml;ller cells of group A, B and C were positive for GFAP and GS. Western blots indicated that ATF4 protein in group A, B and C increased obviously compared with the control group (q=0.293, 0.754,0.484;P＜0.05). Conclusion High glucose can increase the expression of ATF4 protein and cause endoplasmic reticulum stress in retinal M uuml;ller glia cells in vitro.
Citation： 郭敬,柯敏. Effect of high glucose on the expression of activating transcription factor 4 in cultured retinal Müller glia cells. Chinese Journal of Ocular Fundus Diseases, 2012, 28(5): 498-500. doi: Copy