Objective To observe the degradation regulation of ubiquitinproteasome inhibitor nuclear factor kappa;B（NF- kappa;B）and its inhibitory signal protein I kappa;B kinase in earlier period diabetic retinopathy（DR）,and the effects on retinal ganglion cells (RGC) apoptosis.Methods Forty healthy adult Wistar rats were randomly divided into control (group A）,DR（group B）,DR+lowconcentration MG132 treated （group C）and DR+high concentration MG132 treated（group D）groups,10 rats in each group.After 6 and 8 weeks,the results of body masses and fasting blood glucose (FBG) were detected,the expression of NF- kappa;B and I kappa;B were observed by immunohistochemistry respectively.RGC apoptosis was assessed by the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick-end labelling (TUNEL) method.Results The expression of NF- kappa;B was upregulated in group B compared with group A,its expression decreased in group D compared with group B; but the expression of I kappa;B was contrary to NF- kappa;B; RGC apoptosis was followed a similar pattern with the expression of NF- kappa;B; the differences among them were statistically significant (P＜0.01).Compared the expression of NF- kappa;B，I kappa;B and RGC apoptosis in group C and D, there were no statistically significant differences(P＞0.05).Conclusion Ubiquitin-proteasome inhibitor MG132 can block the activation of NF- kappa;B,inhibit ubiquitination of I kappa;B degradation and RGC apoptosis.
Citation： 吴燕,吕红彬,蒋玲,廖洪霞. Effects of proteasome inhibitor MG132 nuclear factorkappa B pathway and retinal ganglion cells apoptosis in rats with diabetic retinopathy. Chinese Journal of Ocular Fundus Diseases, 2010, 26(2): 165-168. doi: Copy